Association study of the polymorphism of partial intron 1 in ABO gene and ABO blood group with Type 2 Diabetes Mellitus Risk in a Zhejiang Han Chinese population

【摘要】：Aims/introduction:Previous studies have observed an association between ABO blood groups and risk for many diseases including metabolic diseases.However,it is unclear whether there is such an association with type 2 diabetes mellitus in the Zhejiang Han Chinese population.In this study,possible relationships between ABO blood groups and partial intron 1 SNPs in ABO gene in patients with type 2 diabetes were investigated.Materials and Methods:ABO blood group type were examined in 196 type 2 diabetes patients and 167 healthy controls(all Zhejiang Han Chinese) using the tube agglutination test,and ABO SNPs were examined in 223 type 2 diabetes patients and 202 healthy controls using the DNA sequencing methods.Results:Spearman correlation analysis and chi-square tests found no association between ABO blood group and type 2 diabetes patients(P=0.963,P=0.770,respectively).The authors analyzed the partial intron 1 and also found 11 SNPs.However,there were no significant differences in the allele frequencies and genotype frequencies between healthy controls and type 2 diabetes patients(P0.05).Additionally,pairwise linkage disequilibrium analysis found no significant haplotype.Conclusions:In the present study,the ABO blood group was not statistically significantly associated with the risk of type 2 diabetes in Zhejiang Han Chinese patients.The allele frequencies,genotype frequencies,and haplotypes of 5 SNPs in intron 1 of the ABO gene showed no correlation to type 2 diabetes.