Comparision of two sequencing methods in the complete mitochondrial genome of Epeorus sp.and the gene arrangement of Epeorus sp.

【摘要】：正Next-generation sequencing(NGS) approach via Long-Range PCR and the classical sequencing via short fragments amplification can successfully obtain the mitochondrial genome of insects.In this study,we compare two sequencing methods in Epeorus sp.(Ephemeroptera, Insecta).We found the NGS is more convenient,cheaper and faster.The complete mtDNA genome is 15,338 bp long and contains 38 genes,and all genes are similar to Parafronurus youi with extral tRNA~(Met).The nucleotide composition of the genome is highly A+T biased(64.7%).A total of 1771 bp of non-coding sequences were observed at 31 inter-genes.The overall A+T content of control region(72.7%),which is similar to other studied mayflies.All PCGs in the mitochondrial genome of Epeorus sp.initiate with the ATD start codon(ATG,ATC,ATT or ATA) and GTG,and terminated with TAA or TAG.