Heat shock protein 90 enhances rat mesenchymal stem cells migration via PI3K/Akt and ERK1/2 pathways
【摘要】:正Objective To investigated whether the Hsp90 would be involved in the migration of mesenchymal stem cells.Methods Rat bone marrow-derived mesenchymal stem cells were harvested from femora and tibia by density gradient eentrifugation.The espremiental was divided five groups:control group,Hsp90αgroup,siRNAHsp90αgroup,17-AAG group,wortmannin group,U0126 group.The ability of MSCs cell migration is determined using the wound healing assay and transwell assay.The activity of matrix