Kruppel-like factor 4 promotes differentiation of endothelial progenitor cells into endothelial cells by up-regulating endothelial nitric oxide synthase
【摘要】:正Objective This present study was designed to investigate whether transcription factors,Krppel-like factor 4(KLF4) is involved in the differentiation of EPCs derived from bone marrow(BM-EPCs) into endothelial cells(ECs). Methods Mononuclear cells were obtained from Sprague-Dawley rats bone marrow by Ficoll density gradient centrifugation and cultured with medium DMEM/F12 in culture dishes,the secondary attached cells were characterized as adherent cells which were double positive for Dil-ac-LDL uptake and lectin binding by direct fluorescent staining demonstrated under a laser