A combination of ultrasonic assisted extraction with RRLC-QQQ method for the determination of artemisinin in the Chinese herb Artemisia annua L.

【摘要】：正To develop and validate an efficient method for extraction and analysis of artemisinin from the plant samples of Artemisia annua L.by rapid resolution liquid chromatography triple quadrupole mass spectrometry(RRLC-QQQ).Following ultrasound-assisted extraction(USE),RRLC-QQQ was utilized to separate and determine artemisinin from the plant sample of Artemisia annua L..The LC separation,QQQ-MS detection and multiple reaction monitoring(MRM) mode were optimized,and the method validation concluding selectivity,calibration,accuracy and precision,and recovery were also evaluated.LC separation was performed with an isocratic elutes of 20%of methanol/water(10 mmol/L ammonium acetate,pH 4.0) on a C_(18) column.The triple quadrupole MS detection was carried out under MRM mode of precursor ion[M+H]~+→fragment ions m/z 265.1 and m/z 247.2.LOD and LOQ of artemisinin were 0.20 and 0.75 ng/mL,respectively.The intra- and inter-day precision did not exceed 3.71%,and the deviation of the intra- and inter-day mean values did not exceed±7.50. The average recoveries for artemisinin ranged from 92.45 to 103.8%with RSD from 2.47 to 2.79%.