182味中药乙醇提取物对酪氨酸酶的抑制作用(英文)

【摘要】：正 OBJECTIVE To observe 182 Chinese crude drugs for inhibitory activity on tyrosinase. Methods Tyrosinase inhibitory activity was determined by the dopachrome method using L-DOPA as substrate and the amount of dopachrome in the reaction mixture was measured as absorbance at 475 nm. Results The 80% ethanolic extracts of 182 Chinese crude drugs were examined for inhibitory activity on tyrosinase. Nine Chinese crude drugs (Glycyrrhiza glabra L., Melaphis Chinese(Bevl.) Baker, Cryptotympana atrata Fabricius, Paeonia suffruticosa Andr., Sophora flavescens Ait., Spirea thunbegii Sieb. et Bl., Xanthium sibiricum Patr., Morus alba L., Rheum Palmatum L.) showed a potent inhibition of tyrosinase compared with positive control arbutin (P0.01), and 11 Chinese crude drugs (Artemisia anomala S. Moore, Hemistepta lyrata Bunge, Lycum Chinese Mill., Dipsacus asper wall., Gastrodia elata Bl., Forsythia suspensa (Thunb.) Vahl., Acer ginnala Maxim., Acer ginnala Maxim., Glycyrrhizza Glycyrrhiza uralensis Fish, Patrinia sabiosaefolia fisch., Buxus sinica (Rehd. et Wils.) Ching, Lonicerajaponica Thunb.) showed no deference compared with positive control arbutin (P0.05). Conclusion These results suggest that 20 Chinese crude drugs (Glycyrrhiza glabra L., Melaphis Chinese (Bevl.) Baker, Cryptotympana atrata Fabricius, Paeonia suffruticosa Andr., Sophora flavescens Ait., Spirea thunbegii Sieb. et Bl., Xanthium sibiricum Patr., Morus alba L., Rheum Palmatum L., Artemisia anomala S. Moore, Hemistepta lyrata Bunge, Lycum chinens is Mill., Dipsacus asper wall., Gastrodia elata Bl., Forsythia suspensa (Thunb.) Vahl., Acer ginnala Maxim., Acer ginnala Maxim., Glycyrrhizza Glycyrrhiza uralensis Fish, Patrnia sabiosaefolia Fisch., Buxus sinica (Rehd. et wils.) Ching, Lonicera japonica Thunb.,) inhibit tyrosinase and could be used as a whitening agent for the skin.