收藏本站
收藏 | 手机打开
二维码
手机客户端打开本文

Effects of Gossypium herbaceam L.extracts(GHL)on the inflammatory process induced by Aβin the brain of rats

【摘要】:正Objects To observe the effects of Gossypium herbaceam L.extracts(GHL) on the inflammatory process induced by Aβin the brain of rats.Methods The rats model of AD were established through i.c.v.injection of Aβ25?35 10μg,and then GHE 35、70、140 mg/kg and donepezil O.6mg/kg were continuously administrated for 14 days.After that,Morris water maze test and step through task were performed to evaluate the learning and memory abilities of rats.Furthermore,the antioxidant activity of GHE was tested by chemistry colorimetry.The cell Viability and apoptosis were evaluated by Nissl and TUNEL staining methods.Some cytokines related to inflammation were analyzed by EMSA,western blotting,ELISA and immunohistochemisty analysis.Results Fingerprint of GHE showed that the main compositions were flavanoids including quercetin.The latency of Morris water maze in AD model group was 2.3 times longer than that in the control group.The GHE 70 mg/kg,GHE 140 mg/kg and donepezil could significantly shorten the escape latencies,and reduce the numbers of errors in step through task(P0.01,P0.05).Additionally,there were obvious neuronal impairments and increased apoptosis in hippocampus of rats in AD model group,and GHE showed significant neuroprotective effects.Moreover,GHE could obviously enhance GSH-Px and CAT levels in hippocampus(P0.05).IL-1βconcentration in hippocampus of rats in model group was 7 times higher than that in the control group,while the expression of endogenous IL-1 receptor antagonist (IL-1RA) in model group was 2 times higher than that in the control group.These might suggest that the activity of endogenous anti-inflammatory system was activated by inflammatory process in model group.GHE and donepezil could enhance the expressions of IL-1RA in hippocampus.In the present study,the ratio of IL-1RA/IL- 1βwas used to evaluate the anti-inflammatory effects of medicine,and the orders of anti-inflammatory effects were shown as following:donepezil(P0.01)GHE 70 mg/kg(P0.01)GHE 140 mg/kg(P0.05).GHE 140 mg/kg and GHE 70 mg/kg could effectively increase the expression of IκB,and inhibit the Aβ_(25-35) induced translocation and activation of NF-κB in nucleus in hippocampus of rats.Conclusions GHE was an ethical herb used by Uygur people in Xinjiang,China,to treat mental retardation,and was recorded in China's Ministry of Health,Drug Standards -Uighur medicine volumes.In the present study,it has been proved that GHE could provide obvious anti-inflammatory and neuroprotective effects.It could block cytokines accumulation processes by increasing the ratio of IL-1 RA / IL-1βand inhibiting NF-κB activation,and ameliorate learning and memory impairment.

知网文化
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978