收藏本站
收藏 | 手机打开
二维码
手机客户端打开本文

AtRAN1 is involved in endosperm development through its imprinting effect

【摘要】:正AtRAN1 belongs to RAN GTPase family in Arabidopsis thaliana.In our study,we found AtRAN1 have a relatively specific expression in gametophytes,embryos and endosperm at reproductive growth stage of Arabidopsis.The AtRAN1 knock-down homozygote had smaller flowers and yielded smaller seeds than WT.In mature AtRAN1 knock-down mutant embryo,cell size instead of cell number was significantly reduced compared with WT,indicating AtRAN1 may take part in seed development.In reciprocal cross,when AtRAN1 knock-down line as female parent to be pollinated with WT,the cross yielded aborted seeds with relatively normal maternal seed coats,but,there was nearly no cellularization happened in endosperm in this kind of aborted seeds.To the contrary,when AtRAN1 knock-down plant acted as male contributor to pollinate WT,the seeds had very early cellularization,were even smaller than either WT or homozygote small seeds.The reciprocal cross of AtRAN1 promoter driving GUS line(pAtRAN1∷GUS) and WT showed that the expression of AtRAN1 from male parent was inhibited at the early developmental stage of seeds,which showed a sign of gene imprinting.These indicated in seed development AtRAN1 may execute its parent-of-origin effect through gene imprinting and control its specific expression in endosperm.At transcription level,the WRKY10 transcription factor(AtMINISEED3) had an extremely higher expression in the aborted seeds and the ectopic higher expression could be down regulated to normal level by pollinate AtMINISEEDS knock out mutant pollens,the aborted seeds phenotype could be recovered too.In contrast, AtMINISEED3 had a lower expression than WT in the small,early cellularization seeds from WT crossed with AtRAN1 knock-down mutant,indicating that the abortion seed phenotypes were AtMINISEED3 related. Conclusion:In conclusion,our results demonstrate that AtRAN1 is an important imprinting factor that is involved in endosperm development probabely through regulating the expression of AtMINISEED3 that regulates the cellularization of endosperm.

知网文化
【相似文献】
中国重要会议论文全文数据库 前10条
1 ;Mechanism of light control of plant development[A];第七届全国光生物学学术会议论文摘要集[C];2010年
2 陈雁;;PAQR family members in cell signaling and development[A];第三届细胞结构与功能的信号基础研讨会论文摘要[C];2010年
3 林鑫华;;Regulation of Wnt signaling and distribution during development[A];中国遗传学会模式生物与人类健康研讨会会议论文集[C];2010年
4 ;Molecular mechanism of G-protein-regulated chloroplast development[A];植物分子生物学与现代农业——全国植物生物学研讨会论文摘要集[C];2010年
5 ;Involvement of CDR3 gene in the regulation of heavy metal resistance as well as seed development and flowering in Arabidopsis[A];第十一届全国植物基因组学大会摘要集[C];2010年
6 ;MSC p43 required for axonal development in motor neurons[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
7 ;The role of Tissue Factor in embryonic development[A];2010·第一届斑马鱼学术研讨会论文摘要集[C];2010年
8 熊敬维;;Role of tln1 in zebrafish cardiovascular development.[A];中国遗传学会模式生物与人类健康研讨会会议论文集[C];2010年
9 ;Periodic Review of Tianmu Mt.Biosphere Reserve:Foster Dialogue and Concertation among Biodiversity Stakeholders[A];中国生物多样性保护与研究进展Ⅶ——第七届全国生物多样性保护与持续利用研讨会论文集[C];2006年
10 ;Dietary modulation of omasal epithelium development in weanling goats[A];中国生理学会2007年消化内分泌生殖学术研讨会论文摘要汇编[C];2007年
中国博士学位论文全文数据库 前4条
1 刘相国;蛋白质组学技术在人类乳腺癌和玉米胚乳中的应用研究[D];吉林大学;2010年
2 常在;Akt1及其异构体在心脏发育过程中的作用研究[D];南京大学;2011年
3 周飞;关于Akt信号通路在淋巴管发育过程中的功能和相关分子机制的研究[D];南京大学;2011年
4 李小冬;拟南芥IKU2互作蛋白鉴定及同源四倍体分析[D];华中农业大学;2012年
中国硕士学位论文全文数据库 前6条
1 徐敏;壳聚糖及其衍生物进行蛋白质特异性吸附分离的研究[D];武汉理工大学;2010年
2 胡雷;水稻FIS类多梳蛋白基因启动子特性及基因表达分析[D];华中农业大学;2010年
3 汪艳杰;水稻polycomb基因家族甲基化状态初步检测[D];华中农业大学;2010年
4 齐束男;拟南芥种子储藏蛋白质相关因子MIP突变体的筛选和表型分析[D];东北林业大学;2011年
5 梁洪雨;灵长类动物脑中特殊类型细胞基因表达情况的研究[D];东北林业大学;2012年
6 王菲;拟南芥ABA2基因调节种子发育的研究[D];山东农业大学;2012年
中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978