Implementation of two high through-put techniques in a novel application:detecting point mutations in large EMS mutated plant populations

【摘要】：正Background:The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom.Considerable efforts have been invested into research on tomato as a model for berry-fruit plants.With the progress of the tomato sequencing project,reverse genetics becomes an obvious and achievable goal.