Genetic diversity and phylogenetic relationship of kenaf germplasm as revealed by SRAP analysis

【摘要】：正The genetic diversity and phylogenetic relationship of kenaf germplasm were analyzed by SRAP(sequence -related amplified polymorphism) marker system.A worldwide collection of 84 accessions from 26 countries and regions was analyzed.Of 225 SRAP primer combinations tested,26 primer combinations were polymorphic.Analyses of 84 kenaf accessions by the 26 primer combinations have identified a total of 329 reproducible bands,248 of them(75.4%) were polymorphic.The number of polymorphic fragments detected by an individual primer combination ranged from 5 to 15,with an average of 9.54 bands per primer.DNA fragment sizes were between 300-1500bp.Cluster analysis of the SRAP fingerprints clearly separated the 84 accessions into three distinct groups:cultivar,wild and intermediate types.The cultivars were further classified into 4 subgroups.High genetic similarity was found among accessions from the same region,this may be due to that kenaf is a cross-pollinated plant.The result suggests that the SRAP molecular marker can be used to indentify cultivar,measure genetic diversity and phylogenetic relationship in kenaf.