Regulatory roles of β-catenin on the proliferation of hair follicle stem cells

【摘要】：正β-catenin is a cytosolic protein that is activated by Wnt signal and responsible for the fate of stem cells during embryogenesis and tumorgenesis.Some data suggested thatβ-catenin might be related to hair follicle development and regeneration involved in the resuscitation of hair follicle stem cells(HFSCs).In previous studies,HFSCs have been observed with superior clonogenicity and proliferative capacity,but the regulatory mechanism is not clear.In this report,we observe the cell growth rates by altering the expression level of p-catenin to elucidate the role ofβ-catenin on the proliferative potency of HFSCs.The data described here show that HFSCs proliferation is significantly increased after treatment with LiC1,which appeared to be mediated by nuclear translocation of stabilizedβ-catenin and activation of responsible cell cycle genes,cyclin D1 and p21.On the contrary,β-catenin knockdown by siRNA led to a decrease of cell growth,accompanied with cyclin D1 downregulation and p21 upregulation.Our results demonstrate that p-catenin is an essential regulator in the control of HFSCs proliferation by regulating cyclin D1 and p21 expression in a canonical Wnt dependent manner and might be a potential therapeutic target for regulation of the yield of keratinocytes derived from HFSCs.