收藏 | 手机打开

Detection of genome-wide copynumber variations in two chicken lines divergently selected for abdominal fat content

Zhang hui  Du zhiqiang  Dong jiaqiang  Wang haixia  Shi hongyan  Wang ning  Wang shouzhi  Li hui  
【摘要】:Background:The chicken(Gallus gallus) is an important model organism that bridges the evolutionary gap between mammals and other vertebrates.Copynumber variations(CNVs) are a form of genomic structural variation widely distributed in the genome.CNV analysis has recently gained greater attention and momentum,as theidentification of CNVs can contribute to a better understanding of traitsimportant to both humans and other animals.To detect chicken CNVs,we genotyped 475 animals derived from two broiler chicken lines divergently selected for abdominal fat content using chicken 60 K SNP array,which is a highthroughput method widely used in chicken genomics studies.Results:Using PennCNV algorithm,we detected 438 and 291 CNVs in the lean and fat lines,respectively,corresponding to271 and 188 CNV regions(CNVRs),which were obtained by merging overlapping CNVs.Out of these CNVRs,99%were confirmed also by the CNVPartition program.These CNVRs covered 40.26 and 30.60 Mb of the chicken genome in the lean and fat lines,respectively.Moreover,CNVRs included 176 loss,68 gain and 27 both(i.e.loss and gain within the same region) events in the lean line,and 143 loss,25 gain and 20 both events in the fat line.Ten CNVRs were chosen for the validation experiment using qPCR method,and allof themwere confirmed in at least one qPCR assay.We found a total of 886 genes located within these CNVRs,and Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analyses showed they could play various rolesin a number ofbiological processes.Integratingthe results of CNVRs,known quantitative trait loci(QTL) and selective sweeps for abdominal fat content suggested that some genes(including SLC9A3,GNAL,SPOCK3,ANXA10,HELIOS,MYLK,CCDC14,SPAG9,SOX5,VSNL1,SMC6,GEN1,MSGN1 andZPAX) may be important for abdominal fat deposition in the chicken.Conclusions:Our study provided a genome-wide CNVR map of the chicken genome,thereby contributingto our understanding of genomic structural variations and their potential roles in abdominal fat content in the chicken.

中国期刊全文数据库 前20条
1 ;Single nucleotide polymorphisms in chicken lmbr1 gene were associated with chicken growth and carcass traits[J];Science in China(Series C:Life Sciences);2007年01期
2 Ying Yang;Feng Sun;Chen Zhang;Hao Wang;Guoyao Wu;Zhenlong Wu;;Hypoxia promotes cell proliferation by modulating E2F1 in chicken pulmonary arterial smooth muscle cells[J];Journal of Animal Science and Biotechnology;2013年03期
3 ;Immunological changes of chicken infected with marek's disease virus[J];The Journal of Northeast Agricultural University(English Edition);1994年01期
4 李宁,陈永福;Ontogenetic expression of chicken growth hormone receptor gene[J];Progress in Natural Science;1995年06期
5 ;Folding patterns of chromatin in chicken erythrocyte studied by using AFM and TEM[J];Chinese Science Bulletin;1998年04期
6 S.Aritomi,N.Fujihara;Production of chicken chimeras by fusing blastodermal cells with electroporation[J];Asian Journal of Andrology;2000年04期
7 Lee Lucy F;Analyzing the H19- and T65-epitopes in 38 kd phosphory-lated protein of Marek's disease viruses and comparing chicken immunological reactions to viruses point-mutated in the epitopes[J];Science in China(Series C:Life Sciences);2004年01期
8 Rong-fa GUAN;Fei LYU;Xiao-qiang CHEN;Jie-qing MA;Han JIANG;Chao-geng XIAO;;Meat quality traits of four Chinese indigenous chicken breeds and one commercial broiler stock[J];Journal of Zhejiang University-Science B(Biomedicine & Biotechnology);2013年10期
9 童俊超,朱榴琴,杨福愉;V92A mutation altered folding propensity of chicken heart apocytochrome c[J];Chinese Science Bulletin;1996年05期
10 包素锦,万柱礼,常文瑞,桂璐璐,梁栋材,凌嵩,李林,许根俊,Simon J. Pilkis;Preliminary crystallographic analysis of recombinant chicken liver fructose-2,6-bisphosphatase[J];Science in China,Ser.B;1996年01期
11 范映辛,朱笠,周筠梅,邹承鲁;Sequential conformation change and activation of chicken liver dihydrofolate reductase in low concentration of guanidine hydrochloride[J];Science in China(Series C:Life Sciences);1997年02期
12 Fuminori Sato,Tomoki Soh,Masa-aki Hattori,Noboru Fujihara;Evaluation of deoxyribonuclease activity in seminal plasmaof ejaculated chicken semen[J];Asian Journal of Andrology;2003年03期
13 ;Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken[J];Science in China(Series C:Life Sciences);2005年02期
14 ;The LTR of endogenous retrovirus ev21 retains promoter activity and exhibits tissue specific transcription in chicken[J];Chinese Science Bulletin;2009年24期
15 ;Adaptability of Fowlpox Virus in Chicken Embryo Passage Cells[J];Animal Husbandry and Feed Science;2011年04期
16 LUO Si-si;XIE Zhi-xun;XIE Zhi-qin;LIU Jia-bo;PANG Yao-shan;DENG Xian-wen;XIE Li-ji;FAN Qing;;Establishment of Indirect ELISA Method for Detection of Penton Protein of Fowl Adenovirus GroupⅠ[J];Animal Husbandry and Feed Science;2012年06期
17 ZHANG Xue-yu;SU Yi-jun;LI Guo-hui;TU Yun-jie;JIANG Yong-jun;YANG Fu-sheng;;Cluster Analysis of Laying Performance and Production Area Ecology of 11 kinds of Chicken[J];Animal Husbandry and Feed Science;2013年01期
18 Mu Yan-shuang;Wang Jia-qiang;Yin Zhi;Jiang Dan-dan;Li Hui;Liu Zhong-hua;;Expression of an Episomal Vector in Developing Chicken Embryo and Chicks[J];Journal of Northeast Agricultural University(English Edition);2013年03期
19 李同元,丁建芳,李林,许根俊;Preparation of monoclonal antibodies against chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and their effects on enzyme activities[J];Science in China(Series C:Life Sciences);1996年04期
20 GAO Gui-sheng;SHI Qiu-mei;ZHANG Yan-ying;SHEN Ping;TANG Sheng-ling;;The Influence of Chinese Medicinal Herb on Chicken Immune Organs and Small Intestinal Mucosa Immune Organization[J];Animal Husbandry and Feed Science;2012年05期
中国重要会议论文全文数据库 前10条
1 ;A novel SNP of visfatin gene and its associations with growth traits in chicken[A];中国动物遗传育种研究进展——第十五次全国动物遗传育种学术讨论会论文集[C];2009年
2 ;The effect of dehydroepiandrosterone on lipogenic gene mRNA expression in cultured primary chicken hepatocytes[A];全国动物生理生化第十一次学术交流会论文摘要汇编[C];2010年
3 ;Studies on the genetic differentiation of fifteen chicken breeds by mtDNA COI gene[A];第十二次全国畜禽遗传标记研讨会论文集[C];2010年
4 ;Use of DNA barcodes to identify indigenous chicken breeds in China[A];中国家禽业——机遇与挑战——第十三次全国家禽学术讨论会论文集[C];2007年
5 ;The effect of dehydroepiandrosterone on cell survival and hepatic lipid lipogenic gene mRNA expression in cultured primary chicken hepatocytes[A];中国生物化学与分子生物学会农业生物化学与分子生物学分会第八次学术研讨会论文集[C];2008年
6 ;Regulation of expression and activity of selenoenzymes by different forms and concentrations of selenium in primary cultured chicken hepatocytes[A];中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会论文集(上册)[C];2011年
7 Pengcheng Jin;Xianwen Wu;Shouzhi Wang;Hui Zhang;Hui Li;;Bioinformatics and tissue expression profile analysis of DNAJB6 gene of chicken[A];第五届全国生物信息学与系统生物学学术大会论文集[C];2012年
8 Bohan Cheng;Li Leng;Hui Li;;Structural and functional analysis of chicken BMP4[A];第五届全国生物信息学与系统生物学学术大会论文集[C];2012年
9 SHU Jingting;XU Wenjuan;SHAN Yanju;SONG Chi;ZHU Wanqi;ZHANG Xueyu;LI Huifang;;Association Analysis of PGC-1αGene with Skeletal Muscle Fibre Types in Chicken[A];中国畜牧兽医学会家禽学分会第九次代表会议暨第十六次全国家禽学术讨论会论文集[C];2013年
10 ;Newly cloned chicken Smad3 is down-regulated by myostatin in chicken embryonic myoblasts[A];中国细胞生物学学会第八届会员代表大会暨学术大会论文摘要集[C];2003年
中国博士学位论文全文数据库 前1条
1 WAEL M.K.ELFEIL (维尔);鸭、鹅PRRs的克隆、分析及分布研究[D];吉林大学;2012年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978