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Gene expression profiling of different development proglottids in Moniezia expansa using cDNA microarray

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【摘要】:正The gene expression profilings in different proglottids of M.expansa were analysed using cDNA microarray.A total of 4,056 spots including full length and partial complementary DNAs representing novel,known,and control genes were studied.Results showed that 55 genes in immature proglottids,134 genes in mature proglottids and 103 genes in gravid proglottids were up-regulated,and that 7 genes in immature proglottids,68 genes in mature proglottids and 78 genes in gravid proglottids were down-regulated compared with control(scolex-neck proglottids).Major function of these genes were about metabolism,transport,protein biosynthesis,transcription factors,apoptosis,cell differentiation,cell communication and nucleic acid binding protein-related.The changes of some genes,such as UBE2A, Cavβ,RAD51,DAZ,PKAc and 2 unknown genes were confirmed by Real-time quantitative Polymerase Chain Reaction(RT-PCR).The complete microarray data set has been deposited in the NCBI Gene Expression Omnibus,GEO Series accession number GSE13982.Results provide a frame of gene expression changes in different development proglottids of M.expansa,which may be helpful in understanding the pathogenesis of the tapeworm and studying its genes concerned with reproductive organs development.

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