Adhesion of probiotic strains to endometrial epithelial cell and interaction with endometritis-associated pathogens

【摘要】：正Aims—To investigate in vitro property of probiotics to impair the adhesion of bacteria that usually associated with uterine infection in cattle.Methods and Results—The adherence of several probiotics,i.e.,Enterococcus faecalis,Lactobacillus casei,Lactobacillus brevis,and Bacillus cereus,that isolated from cervical mucus in health cattle to hircine endometrial epithelial cell lines were investigated. The results indicate that all these strains exhibited different adhesion levels.Furthermore,the inhibitory effects of these probiotics organisms on the adherence of two endometritis-associated pathogens, Escherichia coli and Staphylococcus aureus,were determined.A significant decrease in the number of adhering pathogens was observed,using either preincubation,postincubation or coincubation of the pathogens with probiotics strains.However,although the level of adhesion of B.cereus to endometrial epithelium was significantly higher than those of E.Faecalis and L.brevis,there was no significant difference of the adherence inhibition of two pathogenic bacteria to epithelial cells among three probiotics. Conclusion—These probiotics strains could be used as prevent colonization of the uterine tract by endometritis-associated pathogens.Significance and Impact of the Study—This is the first report of probiotics to inhibit the adhesion of endometritis-associated bacterial pathogens to endometrial epithelium, which shed light on the prevention and treatment of endometritis in cattle.