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In Situ Structure of Intestinal Apical Surface Reveals Nanovilli on Microvilli

Hao Zhu  Meijing Li  Ruixue Zhao  Ming Li  Zhaodi Jiang  Yongping Chai  Zhiwen Zhu  Yihong Yang  Wei Li  Zhongyun Xie  Xiaomin Li  Kexin Lei  Wanzhong He  Xueming Li  欧光朔  
【摘要】:正Microvilli are actin bundle-supported membrane protrusions essential for absorption,secretion,and sensation.Microvilli defects cause human diseases,including gastrointestinal disorders and inherited deafness; however,mechanisms controlling microvilli formation and organization remain unclear.Here,we study microvilli by vitrifying the C.elegans larvae and mouse intestinal tissues with high-pressure freezing,thinning them by cryo-focused ion beam milling,cryo-electron tomography,and sub-tomogram averaging.

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