Direct synthesis of EM-visible gold nanoparticles on genetically encoded tags for single-molecule visualization in cells

【摘要】：正Single-molecule detection of proteins in cells with genetically encoded tags for electron microscopy (EM) has been a long-awaited but unimplemented tool for cell biologists. Here, we report an approach for directly synthesizing EM-visible gold nanoparticles (AuNPs) on cysteinerich tags for single-molecule detection in cells. We first uncovered an auto-nucleation suppression mechanism that allows specific synthesis of AuNPs on isolated cysteine-rich tags. We next exploited this mechanism to develop an approach for single-molecule detection of proteins in

下载App查看全文

下载全文更多同类文献论文一键排版服务>>

PDF全文下载

CAJ全文下载

(如何获取全文？欢迎：购买知网充值卡、在线充值、在线咨询)

CAJViewer阅读器支持CAJ、PDF文件格式，AdobeReader仅支持PDF格式

	【相似文献】
	【相似文献】

中国期刊全文数据库

前19条

1	;COVER STORY[J];Journal of Energy Chemistry;2014年02期
2	EICHLER H.J,GAD G.M.A,RHEE H;Raman crystal lasers in the visible and near-infrared[J];Journal of Zhejiang University Science;2003年03期
3	;Evaluate the inactivation efficiency of HCV byMB/visible light using RTPCR[J];中国输血杂志;2001年S1期
4	;UV－visible　and　Electric　Property　Analysis　of　PET　Bombarded　by　Different　Charge　State　Ar[J];1996 Annual Report of Institute of Modern Physics,the Chinese Academy of Sciences & National Laboratory of Heavy Ion Accelerator,LanZhou;1996年00期
5	Nicolaas Bom,Charles T. Lancee,Nico de Jong,Cornells J. Slager;SPECIAL TRANSDUCERS[J];中国超声医学杂志;1988年S1期
6	苏根博,贺友平,吴柏昌;A New Organic Nonlinear Optical Crystal-Cyclourea Compound(CNU)[J];人工晶体;1988年Z1期
7	刘立人;OBSERVABLE IMAGING AND CHARACTER RECOGNITION OF PHASE OBJECTS BY CODED-SPECTRUM DIFFRACTION INTERFEROMETRY[J];Science Bulletin;1988年08期
8	;Synthesis of ZnO nanorods and their optical absorption in visible-light region[J];Rare Metals;2006年01期
9	Meng-Jie Chang;Wen-Na Cui;Jun Liu;Kang Wang;Hui-Ling Du;Lei Qiu;Si-Meng Fan;Zhen-Min Luo;;Construction of novel TiO_2/Bi_4Ti_3O_(12)/MoS_2 core/shell nanofibers for enhanced visible light photocatalysis[J];Journal of Materials Science & Technology;2020年01期
10	Jian Wang;Jian Zhang;Shing Bo Peh;Guoliang Liu;Tanay Kundu;Jinqiao Dong;Yunpan Ying;Yuhong Qian;Dan Zhao;;Cobalt-containing covalent organic frameworks for visible light-driven hydrogen evolution[J];Science China(Chemistry);2020年02期
11	YOU WU;ZHIWEN LI;KAH-WEE ANG;YUPING JIA;ZHIMING SHI;ZHI HUANG;WENJIE YU;XIAOJUAN SUN;XINKE LIU;DABING LI;;Monolithic integration of MoS_2-based visible detectors and GaN-based UV detectors[J];Photonics Research;2019年10期
12	Ruifen Wang;Shengli An;Jing Zhang;Jinling Song;Fuming Wang;;Existence form of lathanum and its improving mechanism of visible-light-driven La-F co-doped TiO_2[J];Journal of Rare Earths;2020年01期
13	Zhe Feng;Lin Zeng;Qingle Zhang;Shifeng Ge;Xinyue Zhao;Hongjun Lin;Yiming He;;In situ preparation of g-C_3N_4/Bi_4O_5I_2 complex and its elevated photoactivity in Methyl Orange degradation under visible light[J];Journal of Environmental Sciences;2020年01期
14	Hong-Tao Zhang;Li-Jun Gu;Xiang-Zhong Huang;Rui Wang;Cheng Jin;Gan-Peng Li;;Synthesis of indol-3-yl aryl ketones through visible-light-mediated carbonylation[J];Chinese Chemical Letters;2016年02期
15	Chenhui Hu;Lihong Zhang;Liyuan Cheng;Jing Chen;Wenhua Hou;Weiping Ding;;A comparison of H~+-restacked nanosheets and nanoscrolls derived from K_4Nb_6O_(17) for visible-light degradation of dyes[J];Journal of Energy Chemistry;2014年02期
16	;A facile way to prepare visible light driven tin oxide based photoanode and its photoelectrochemical water splitting properties[J];Chinese Science Bulletin;2012年32期
17	Karunamoorthy Saravanakumar;Subramani Muthupoongodi;Velluchamy Muthuraj;;A novel n-CeO_2/n-CdO heterojunction nanocomposite for enhanced photodegradation of organic pollutants under visible light irradiation[J];Journal of Rare Earths;2019年08期
18	贾永坡;叶玮琳;田崇文;全相印;王嘉琪;宋占伟;郑传涛;;Numerical analysis on an OOK-NRZ visible light communication system based on a single white LED[J];Optoelectronics Letters;2011年05期
19	刘从义;敬忠良;肖刚;杨波;;Feature-based fusion of infrared and visible dynamic images using target detection[J];Chinese Optics Letters;2007年05期

中国重要会议论文全文数据库

前10条

1	何万中;;Direct synthesis of EM-visible gold nanoparticles on genetically encoded tags for single-molecule visualization in cells[A];第六届全国冷冻电子显微学与结构生物学专题研讨会论文集[C];2019年
2	Jian Luo;Zhifeng Cheng;Zeping Zhang;Xiaolong Zhang;Saiying Hou;Cheng Cheng;;Preliminary Analysis of the Effective Ion Charge in J-TEXT Tokamak[A];第十六届全国等离子体科学技术会议暨第一届全国等离子体医学研讨会会议摘要集[C];2013年
3	;Non-concentric nanocrescent/nanodisk structure: Local field enhancement in the visible and near-infrared light regions[A];中国光学学会2011年学术大会摘要集[C];2011年
4	;A New One-Dimensional Germanium Thioantimonate with Photocatalytic Property[A];中国化学会第六届全国结构化学学术会议论文摘要[C];2012年
5	;Photocatalytic hydrogen production over metal-doped Cd_xZn_(1-x)S under visible light[A];2008 International Hydrogen Forum Programme and Abstract[C];2008年
6	林泽文;Kunji Chen;Pengzhan Zhang;Jun Xu;Tao Wu;Wei Li;Huafeng Yang;Xinfan Huang;;A visible light-emitting device made by P-doped silicon oxynitride films[A];第十一届全国硅基光电子材料及器件研讨会论文摘要集[C];2016年
7	Bao Zhang;Yanming Zhao;Yaqing Feng;;Constructing metal-porphyrinic framework-based composite photocatalysts with visible-light-driven photocatalytic activities[A];中国化学会第四届卟啉与酞菁学术研讨会论文集[C];2017年
8	李金龙;;Fabrication of nitrogen-doped meso-porous TiO_2 layer with higher visible photocatalytic activity by plasma-based ion implantation[A];第二届中国科学院博士后学术年会暨高新技术前沿与发展学术会议程序册[C];2010年
9	HOU Zhao-fei;ZHAO Wei;YU Feng;;The Visible Light Imaging Detection Performance of Spaced-target[A];2015光电防御技术学术研讨会摘要集[C];2015年
10	李青瑞;李亚波;黄萌萌;Jung Keun Kim;吴养洁;;An Approach to Phosphinylated Coumarins under Green LED Visible-Light[A];第十五届全国光化学学术讨论会会议论文集[C];2017年

中国博士学位论文全文数据库

前2条

1	Segomotso Bagwasi;[D];华东理工大学;2012年
2	Muhammad Nasir;[D];华东理工大学;2013年

中国硕士学位论文全文数据库

前5条

1	Do Duc Tung;[D];上海交通大学;2015年
2	Mamdouh Dulaym Alanazi;[D];北京化工大学;2017年
3	邵小丽;微博语码转换的修辞研究[D];烟台大学;2016年
4	MOHAMMED ALFAIFI;碘掺杂TiO_2/g—C_3N_4的制备及其光催化性能研究[D];北京化工大学;2016年
5	何瑞珍;基于GIS林业管理信息系统研究[D];河南农业大学;2004年

中国重要报纸全文数据库

前1条

1	山东省嘉祥县职业中专钱善华;读者来信[N];中国电脑教育报;2004年

	快捷付款方式		订购知网充值卡	订购热线	帮助中心
	微信支付支付宝银行卡	知网卡在线购卡更多>>	免费送卡上门银行汇款购卡邮局汇款购卡	400-819-9993 010-62982499 010-62783978	常见问题在线咨询阅读器下载	充值中心知网卡新手指南