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Direct synthesis of EM-visible gold nanoparticles on genetically encoded tags for single-molecule visualization in cells

【摘要】:正Single-molecule detection of proteins in cells with genetically encoded tags for electron microscopy (EM) has been a long-awaited but unimplemented tool for cell biologists. Here, we report an approach for directly synthesizing EM-visible gold nanoparticles (AuNPs) on cysteinerich tags for single-molecule detection in cells. We first uncovered an auto-nucleation suppression mechanism that allows specific synthesis of AuNPs on isolated cysteine-rich tags. We next exploited this mechanism to develop an approach for single-molecule detection of proteins in

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