收藏 | 手机打开

Rapid mapping of a stripe rust resistance gene in wheat cultivar Zhongliang 31 using BSA combined with high-throughput SNP genotyping arrays

Liu Huan  Su Wenwen  Chao Kaixiang  Li Qian  Yue Weiyun  Wang Baotong  Li Qiang  
【摘要】:Stripe rust,caused by Puccinia striiformis f.sp.tritici(Pst),is one of the most important diseases of wheat(Triticum aestivum L.) and causes substantial yield losses in many wheat-growing regions of the world.Identifying and utilization of new resistance genes is the most effective way for achieving durable disease control.Zhongliang 31,a common wheat highly resistant to Chinese predominant Pst races both in the seedling and adult-plant stages,has been grown widely in Longnan region in Gansu Province where is the hotspot for stripe rust pathogen.Inheritance analysis of F_2 and F_(2:3) populations indicated that the stripe rust resistance of Zhongliang 31 is controlled by a single dominant gene,temporarily designated as Yrzl31.Bulked segregant analysis(BSA) combined with wheat 660 K single nucleotide polymorphism(SNP) array showed 2050 of 9118 polymorphic SNPs located on chromosome 2 B.141 kompetitive allele specific PCR(KASP) selected from the 2050 SNPs and 165SSR markers on chromosome 2 B were used to map Yrzl31.Linkage analysis indicated that 49 markers were linked to Yrzl31 and the genetic distances of two closest flanking markers KASP AX-111650565 and AX-110196738 were2.8 cM and 4.1 cM,respectively.Also,Yrzl31 was located on wheat chromosome 2 BL.The chromosome location,resistance and molecular tests suggested that Yrzl31 may be a novel gene.Since this gene is present at low frequency in the current wheat germplasms in China,it can be pyramided with other effective stripe rust resistance genes using marker-assisted selection(MAS).

中国期刊全文数据库 前20条
1 XU Xiao-dan;FENG Jing;FAN Jie-ru;LIU Zhi-yong;LI Qiang;ZHOU Yi-lin;MA Zhan-hong;;Identification of the resistance gene to powdery mildew in Chinese wheat landrace Baiyouyantiao[J];Journal of Integrative Agriculture;2018年01期
2 ;Synthetic hexaploid wheat and its utilization for wheat genetic improvement in China[J];遗传学报;2009年09期
3 ;Alien DNA introgression and wheat DNA rearrangements in a stable wheat line derived from the early generation of distant hybridization[J];Science in China(Series C:Life Sciences);2005年05期
4 Zhongfu Ni;Hongjian Li;Yue Zhao;Huiru Peng;Zhaorong Hu;Mingming Xin;Qixin Sun;;Genetic improvement of heat tolerance in wheat:Recent progress in understanding the underlying molecular mechanisms[J];The Crop Journal;2018年01期
5 YUE Ai-qin;LI Ang;MAO Xin-guo;CHANG Xiao-ping;LI Run-zhi;JING Rui-lian;;Single-nucleotide polymorphisms,mapping and association analysis of 1-FFT-A1 gene in wheat[J];Journal of Integrative Agriculture;2017年04期
6 ;Utilization of blue-grained character in wheat breeding derived from Thinopyrum poticum[J];遗传学报;2009年09期
7 CHEN Xue-yan;SONG Guo-qi;ZHANG Shu-juan;LI Yu-lian;GAO Jie;Islam Shahidul;MA Wu-jun;LI Gen-ying;JI Wan-quan;;The allelic distribution and variation analysis of the NAM-B1 gene in Chinese wheat cultivars[J];Journal of Integrative Agriculture;2017年06期
8 ;Change of water use efficiency and its relation with root system growth in wheat evolution[J];Chinese Science Bulletin;2002年22期
9 LI Qiang;WANG Zheng-rui;LI Ding;WEI Jian-wei;QIAO Wen-chen;MENG Xiang-hai;SUN Shu-luan;LI Hui-min;ZHAO Ming-hui;CHEN Xiu-min;ZHAO Feng-wu;;Evaluation of a new method for quantification of heat tolerance in different wheat cultivars[J];Journal of Integrative Agriculture;2018年04期
10 ;Discovery of quantitative trait loci for crossability from a synthetic wheat genotype[J];遗传学报;2011年08期
11 HUSSAIN Iqtidar;KHAN Muhammad Ayyaz;KHAN Ejaz Ahmad;;Bread wheat varieties as influenced by different nitrogen levels[J];Journal of Zhejiang University Science;2006年01期
12 朱其洪,贾旭,王京兆,李海健,张驰,庄家俊,王斌;Obtainment of molecular markers linked to BYDV resistance of wheat by RAPD[J];Chinese Science Bulletin;1996年01期
13 贾旭,庄家骏,胡适全,李海健,聂道泰,俞春江,王剑雄,钱幼亭,周广和;A new way of creating new wheat germplasms resistant to wheat diseases[J];Chinese Science Bulletin;1995年19期
14 ZOU Ya-fei;QIAO Hong-bo;CAO Xue-ren;LIU Wei;FAN Jie-ru;SONG Yu-li;WANG Bao-tong;ZHOU Yi-lin;;Regionalization of wheat powdery mildew oversummering in China based on digital elevation[J];Journal of Integrative Agriculture;2018年04期
15 Yujie LIU;Qiaomin CHEN;Quansheng GE;Junhu DAI;;Spatiotemporal differentiation of changes in wheat phenology in China under climate change from 1981 to 2010[J];Science China(Earth Sciences);2018年08期
16 XIE Jing-zhong;WANG Li-li;WANG Yong;ZHANG Huai-zhi;ZHOU Sheng-hui;WU Qiu-hong;CHEN Yong-xing;WANG Zhen-zhong;WANG Guo-xin;ZHANG De-yun;ZHANG Yan;HU Tie-zhu;LIU Zhi-yong;;Fine mapping of powdery mildew resistance gene PmTm4 in wheat using comparative genomics[J];Journal of Integrative Agriculture;2017年03期
17 LI Meng;TIAN Xiao-hong;LI Xiu-li;WANG Shao-xia;;Effect of Zn application methods on Zn distribution and bioavailability in wheat pearling fractions of two wheat genotypes[J];Journal of Integrative Agriculture;2017年07期
18 Liuping Zhang;Hong Lu;Kun Guo;Shumin Yao;Feng Cui;;Insecticide resistance status and detoxification enzymes of wheat aphids Sitobion avenae and Rhopalosiphum padi[J];Science China(Life Sciences);2017年08期
19 HE Yi;WANG Qiong;ZENG Jian;SUN Tao;YANG Guang-xiao;HE Guang-yuan;;Current status and trends of wheat genetic transformation studies in China[J];Journal of Integrative Agriculture;2015年03期
20 Zhilin Zhu;Xiaomin Sun;Fenghua Zhao;Franz X.Meixner;;Ozone concentrations, flux and potential effect on yield during wheat growth in the NorthwestShandong Plain of China[J];Journal of Environmental Sciences;2015年08期
中国重要会议论文全文数据库 前10条
1 Liu Huan;Su Wenwen;Chao Kaixiang;Li Qian;Yue Weiyun;Wang Baotong;Li Qiang;;Rapid mapping of a stripe rust resistance gene in wheat cultivar Zhongliang 31 using BSA combined with high-throughput SNP genotyping arrays[A];中国植物病理学会2018年学术年会论文集[C];2018年
2 Liang Junchao;Fu Bisheng;Tang Wenbin;Khan U.Nasr;Li Na;Ma Zhengqiang;;Fine mapping of two wheat powdery mildew resistance genes located at the Pm1 cluster[A];第六届全国小麦基因组学及分子育种大会论文集[C];2015年
3 ;Establishment of E genome of Thinopyrum elongatum specific SCAR marker[A];第十一届全国植物基因组学大会摘要集[C];2010年
4 ;Mapping of chromosome regions for grain weight of wheat and their allelic variations[A];第十三届全国植物基因组学大会论文集[C];2012年
5 ZHANG Pei-pei;LI Qing-luo;WANG Xin-hai;LI Zai-feng;LIU Da-qun;;QTL mapping of adult-plant resistance to leaf rust in Romanian wheat line Fundulea 900[A];中国植物病理学会2015年学术年会论文集[C];2015年
6 ;Construction and characterization of three bacterial artificial chromosome libraries of wheat[A];第十一届全国植物基因组学大会摘要集[C];2010年
7 Fangfang Liu;Hongqi Si;Cheng Chang;Haiping Zhang;Chuanxi Ma;;Chromosome reassignment and evolutionary analysis of the Wcs19and Wcor15genes in wheat[A];第六届全国小麦基因组学及分子育种大会论文集[C];2015年
8 Zhang Fuyan;Wu Peipei;Chen Feng;Cui Dangqun;;Molecular characterization of lipoxygenase genes on the chromosome of 4BS in Chinese bread wheat(Triticumaestivum L.)[A];第六届全国小麦基因组学及分子育种大会论文集[C];2015年
9 ;Creation and identification of wheat-barley 2H recombination materials with Isa-H1[A];中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会论文摘要汇编[C];2009年
10 ;Identification and mapping of two powdery mildew resistance genes in common wheat D57[A];第十一届全国植物基因组学大会摘要集[C];2010年
中国博士学位论文全文数据库 前10条
1 Shoaib Ur Rehman;小麦蔗糖非发酵相关蛋白激酶2基因TaSnRK2.9的功能分析[D];中国农业科学院;2018年
2 罗培高;小麦对条锈病、白粉病和衰老抗性的分子细胞生物学研究[D];四川农业大学;2006年
3 李玲玲;[D];甘肃农业大学;2006年
4 Muharam Ali;抗条锈病小麦N95175抗性基因的遗传图谱与克隆[D];西北农林科技大学;2010年
5 Muhammad Ejaz Khan;[D];西北农林科技大学;2013年
6 Mohamad Hesam Shahrajabian;华北平原冬小麦灌溉效应及蒸发、蒸腾的田间蒸渗仪评价[D];中国农业大学;2015年
7 Mamoona Hanif;小麦TaTGW6基因的克隆鉴定和功能标记开发[D];中国农业科学院;2016年
8 王丽芳;旱作小麦产量形成及其对不同覆盖与耕作措施的响应[D];西北农林科技大学;2016年
9 Nadia Khan;不同水分条件下小麦茎秆可溶性糖和农艺性状的遗传解析[D];中国农业科学院;2015年
10 Bakht un Nisa Mangan;[D];西北农林科技大学;2014年
中国硕士学位论文全文数据库 前10条
1 Mamoudou Saidou;小麦新种质N0324抗白粉病基因的染色体定位与分子标记[D];西北农林科技大学;2013年
2 Lise Josephine Gerard;对中国小麦市场未来的研究[D];华东理工大学;2015年
3 韩国强;不同施氮条件下小麦品种的碳氮转移效率研究[D];福建农林大学;2010年
4 郑青焕;21份印度小麦种质品质性状的评价及主要农艺性状分析[D];西北农林科技大学;2016年
5 Shahat Sabet MOHAMED;中埃农业比较研究[D];中国农业科学院;2013年
6 刘占锋;利用作物模拟模型辅助冬小麦限水灌溉决策的研究[D];河北农业大学;2004年
7 杨宁;豆科绿肥—冬小麦轮作提高小麦产量和营养元素含量的效应与土壤机制[D];西北农林科技大学;2012年
8 拉钠(RANA ABDUL SAMAD);[D];华中农业大学;2016年
9 罗怀勇;小麦条锈病、白粉病和叶锈病抗病基因的分子标记[D];四川农业大学;2010年
10 张志良;小麦地方品种红蚰麦抗白粉病基因的发掘和定位[D];南京农业大学;2015年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978