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Molecular mapping of a recessive powdery mildew resistance gene in wheat cultivar Tianxuan 45 using bulked segregant analysis with polymorphic SNPs relative ratio distribution

Chao Kaixiang  Su Wenwen  Wu Lei  Su Bei  Li Qiang  Wang Baotong  
【摘要】:Powdery mildew is a destructive foliar disease of wheat worldwide.Wheat variety ' Tianxuan 45' exhibits resistance to the highly virulent isolate HY5.Genetic analysis of the F_2 and F_(2.3) populations of a Mingxian169/Tianxuan 45 cross revealed that the resistance to HY5 was controlled by a single recessive gene,temporarily designated as PmTx45.A Manhattan plot with the relative frequency distribution of single nucleotide polymorphisms(SNP) was used to rapidly narrow down the possible chromosomal regions of the associated genes.This microarray-based bulked segregant analysis(BSA) largely improved traditional analytical methods.PmTx45 was located in chromosomal bin 4 BL5-0.86-1.00 and flanked by SNP marker AY-110673642 and Intron length polymorphism(ILP) marker ILP-4 B01 G269900 with genetic distances of 3.OcM and 2.6 cM,respectively.Molecular detection in a panel of wheat cultivars using the markers linked to PmTx45 showed that the presence of PmTx45 in commercial wheat cultivar was rare.Resistance spectrum and chromosomal position analyses indicated that PmTx45 may be a novel recessive gene with moderate powdery mildew resistance.This new microarray-based BSA method is feasible and effective and has the potential application for mapping genes in wheat in markerassisted breeding.

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