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The regulation of KCNQ2/3 potassium channel by extracelluar K~+ and pH

【摘要】:正Aim: To observe the effect of extracelluar of K+ and pH on KCNQ2/3 potassium current and explore the mechanism of regulation. Methods: The cRNAs of KCNQ were prepared using RibomaxTM Large Scale RNA Production Systems-SP6 and T7 Kit. Xenopus Oocytes injected with the cRNA of KCNQ2 and KCNQ3 potassium channel by 1:1 ratio were cultured in ND96 solution for 2-3 d. Whole cell KCNQ currents were recorded using TEVC method and to observe the effect of different concentrations of K+ and H+ solutions. Results: (1)The amplitude of KCNQ2/3 current was enhanced by extracellular K+ in concentration-dependent manner. Elevation of external [K+] at a range of 0-15mmol/L potentiated KCNQ2/3 and KCNQ2 currents, and the effect of external [K+] was more significant on KCNQ2 currents than KCNQ2/3 currents. (2) Elevation of extracellular [K+] had obvious effect on the slope of KCNQ2/3 channel's activation curve. When extracellular [K+] was changed from 0 and 12mmol/L, the initial potential of activation was not changed (-60mV), and the half activation voltage was shifted slightly to

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