Establishment and characterization of a cell line,EH-GB2,derived from hepatic metastasis of gallbladder cancer

【摘要】：正Gallbladder cancer is a fatal neoplasia with an extremely low survival rate.Liver invasion and metastasis is the most common causes of death;however,the metastatic mechanism is still unclear,and no effective treatment methods are available.To provide comprehensive and profound approaches in investigating the metastatic mechanism and treat methods,new cell lines derived from liver metastasis are urgently needed.A hepatic metastasis lesion was obtained from a 65 years old patient,and was treated by primary culture method to establish a novel gallbladder cancer cell line.Different in vitro /vivo methods were used to characterize phenotypes of this cell line.The gallbladder cancer cell line was named as EH-GB2,with an about 48 h doubling time.The cell line represents stronger colony formation and migration abilities than control group.The cells have a complicated chromosomal abnormality.EH-GB2 cells showed epithelial-to-mesenchymal transition(EMT) and the mRNA expression levels of E-cadherin and Integrin were decreased,and that of vimentin,Snail,Twist,matrix metalloproteinase-l(MMP-l),and MMP-2 were increased in comparison with control cells.In vivo study,EH-GB2 presents a well tumorigenecity in nude mice. This established gallbladder cancer cell line EH-GB2 is useful for future study of gallbladder cancer development,progression,metastasis,and therapy.