The inhibitory effect of celastrol on the process of brain glioma stem cells' differentiation into vascular endothelial cells

【摘要】：Objective To let brain glioma stem cells differentiate into vascular endothelial cells on certain conditions in vitro for further study of celastrol's inhibiting its differentiation process.Method Brain glioma stem cells were cultured in neural stem cell culture fluid,then cells' shape changes were observed and CD133,Nestin,CD31 and KDR immunofluorescence stainings were used to examine the cells after passage.Brain glioma stem cells were divided into the experimental group and the control group after passage.The experimental group cells were cultured by differentiation culture fluid in anaerobic environment while the control group cells were cultured by ordinary culture fluid in aerobic environment.Cells shape changes were observed by the microscope.After 12 days,CD31 and KDR immunofluorescence stainings were used to examine the cells,and the cells were planted on Matrigel glue to observe the cells shape changes.One part of brain glioma stem cells were differentiated for 72 hours.And then differente concentrations celastrol fluids such as 0.04μg/ml、0.2μg/ml、1.0μg/ml、5.0μg/ml、25.0μg/ml、 125.0μg/ml were added to culture the cells for 48 hours.Cells were continued to differentiated for 7 days.Then cells shapes were observed and CD31,KDR immunofluorescence staining were used.The other part of brain glioma stem cells were planted on the Matrigel glue,different concentrations celastrol fluids were added like the front steps.We observed whether tubular structures could form.Conclusion The brain glioma stem cells which were cultured by differentiation culture fluid in anaerobic environment in vitro for 12 days could differentiate into vascular endothelial cells.The celastrol can inhibit the process of the brain glioma stem cells' differentiation into vascular endothelial cells significantly in certain concentration range.