收藏本站
收藏 | 论文排版

The inhibitory effect of celastrol on the process of brain glioma stem cells' differentiation into vascular endothelial cells

【摘要】:Objective To let brain glioma stem cells differentiate into vascular endothelial cells on certain conditions in vitro for further study of celastrol's inhibiting its differentiation process.Method Brain glioma stem cells were cultured in neural stem cell culture fluid,then cells' shape changes were observed and CD133,Nestin,CD31 and KDR immunofluorescence stainings were used to examine the cells after passage.Brain glioma stem cells were divided into the experimental group and the control group after passage.The experimental group cells were cultured by differentiation culture fluid in anaerobic environment while the control group cells were cultured by ordinary culture fluid in aerobic environment.Cells shape changes were observed by the microscope.After 12 days,CD31 and KDR immunofluorescence stainings were used to examine the cells,and the cells were planted on Matrigel glue to observe the cells shape changes.One part of brain glioma stem cells were differentiated for 72 hours.And then differente concentrations celastrol fluids such as 0.04μg/ml、0.2μg/ml、1.0μg/ml、5.0μg/ml、25.0μg/ml、 125.0μg/ml were added to culture the cells for 48 hours.Cells were continued to differentiated for 7 days.Then cells shapes were observed and CD31,KDR immunofluorescence staining were used.The other part of brain glioma stem cells were planted on the Matrigel glue,different concentrations celastrol fluids were added like the front steps.We observed whether tubular structures could form.Conclusion The brain glioma stem cells which were cultured by differentiation culture fluid in anaerobic environment in vitro for 12 days could differentiate into vascular endothelial cells.The celastrol can inhibit the process of the brain glioma stem cells' differentiation into vascular endothelial cells significantly in certain concentration range.

知网文化
【相似文献】
中国重要会议论文全文数据库 前10条
1 ;Boron uptake of glioma stem/progenitor cells in vitro and in vivo[A];中国抗癌协会神经肿瘤专业委员会第八届学术会议论文集[C];2011年
2 ;The inhibitory effect of celastrol on the process of brain glioma stem cells' differentiation into vascular endothelial cells[A];中国抗癌协会神经肿瘤专业委员会第八届学术会议论文集[C];2011年
3 Chae-Yong Kim;Young-Hoon Kim;Seung Hee Cho;Soo Jung Lee;Seung Ah Choi;Ji Hoon Phi;Seung-Ki Kim;Kyu-Chang Wang;Byung-Kyu Cho;;Growth-inhibitory effect of neurotrophin-3-secreting adipose tissue-derived mesenchymal stem cells on the D283-MED human medulloblastoma cell line[A];中国抗癌协会神经肿瘤专业委员会第八届学术会议论文集[C];2011年
4 Takuichiro Hide;Tatsuya Takezaki;Hideo Nakamura;Keishi Makino;Jun-ichi Kuratsu;Toru Kondo;;Glioblastoma-initiating cells transformed from oligodendrocyte precursor cells are sensitive for the combination of Cox2 inhibitor and EGFR inhibitor[A];中国抗癌协会神经肿瘤专业委员会第八届学术会议论文集[C];2011年
5 易良;;Neurotensin expressed by glioma-initiating cells affects clinical outcome in glioma patients[A];中国医师协会神经外科医师分会第六届全国代表大会论文汇编[C];2011年
6 JJ Yiin;B Hu;J Wu;Y Rao;HI Ma;SY Cheng;;SLIT2 inhibits glioma invasion in the brain[A];中国抗癌协会神经肿瘤专业委员会第八届学术会议论文集[C];2011年
7 Seok-Gu Kang;Hyun-Su Mok;Hyun-Do Shin;Yoon-Kyung Park;Na-Ri Park;Youn Soo Lee;YongKil Hong;;Role of human glioma stroma mesenchymal stem cells in human glioma[A];中国抗癌协会神经肿瘤专业委员会第八届学术会议论文集[C];2011年
8 Won Ho Cho;Byung Kwan Choi;In Ho Han;Seung Heon Cha;;Capsaicin-induced apoptosis in the human glioblastoma U87MG cells via p-38 MAPK and Bcl-2/Bax signaling pathway[A];中国抗癌协会神经肿瘤专业委员会第八届学术会议论文集[C];2011年
9 杨孔宾;刘宾学;胡立;王宁;赵世光;;Role of reactive oxygen species in the apoptosis of rat glioma cells induced by arsenic trioxide[A];中国医师协会神经外科医师分会第六届全国代表大会论文汇编[C];2011年
10 张清波;;An Experimental Study on Functional Response of Gliomas Vasculature to Hypercarbia by Perfusion weighted MR Imaging[A];中华医学会第九次全国核医学学术会议论文摘要汇编[C];2011年
中国博士学位论文全文数据库 前10条
1 于靓霞;间充质干细胞输注治疗重症肌无力的实验研究[D];北京协和医学院;2011年
2 李学元;mTOR信号通路和GOLPH3在人脑胶质瘤中的表达和临床意义[D];山东大学;2011年
3 叶显宗;肿瘤相关巨噬细胞促进胶质瘤干细胞侵袭及其机制研究[D];第三军医大学;2012年
4 柳影;氯化甲基汞抗胶质瘤作用及其分子机制的研究[D];吉林大学;2010年
5 徐硕;胞外酶CD39-CD73-腺苷代谢通路参与恶性胶质瘤免疫抑制及其治疗前景的研究[D];山东大学;2013年
6 王海峰;抑制蛋白酶体功能对脑胶质瘤作用的实验研究[D];吉林大学;2010年
7 张祖萍;脑胶质瘤的表观遗传学研究[D];中南大学;2010年
8 施金龙;人脑胶质瘤中IDH1基因突变及DNA甲基化调控与干细胞相关基因表达[D];复旦大学;2010年
9 张鹏;NKG2D介导的同种异体NK细胞对人脑胶质瘤细胞杀伤效应的实验研究[D];郑州大学;2011年
10 王喆;miRNA-20/106通过抑制TIMP-2表达促进人胶质瘤干细胞侵袭的机制研究[D];第三军医大学;2011年
中国硕士学位论文全文数据库 前10条
1 Tabitha Mlowoka Kayira;[D];东北师范大学;2012年
2 江信宏;康复训练对急性缺血性脑卒中患者循环内皮祖细胞的影响及其意义[D];福建医科大学;2012年
3 黄炜;PEX基因对脑胶质瘤干细胞生物活性影响的实验研究[D];安徽医科大学;2012年
4 尹航;铜绿假单胞菌制剂对大鼠C6胶质瘤治疗作用的实验研究[D];昆明医学院;2010年
5 范菁;阻断HH信号通路对脑胶质瘤细胞增殖抑制与诱导凋亡作用的实验研究[D];浙江大学;2010年
6 黄天翔;AEG-1基因在人脑胶质瘤中的表达及意义[D];中南大学;2010年
7 魏盈胜;WIF-1基因在人脑胶质瘤中的表达及意义[D];中南大学;2010年
8 叶永斌;Ⅰ型γ-分泌酶抑制剂对恶性胶质瘤细胞株的增殖及凋亡的影响[D];南方医科大学;2010年
9 张春;人类疱疹病毒6型与神经胶质瘤关系的研究[D];南京医科大学;2011年
10 严丹方;脑胶质瘤术后调强放疗的近期疗效及生存质量观察[D];浙江大学;2011年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978