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A Fluorogenic Phosphine-Ester Conjugate for Monitoring Protein S-nitrosylation in Endothelial Cells

Shao Shiyi  Chen Bo  Cheng Juan  Wang Chengkun  Shao Lingxiao  Shao Baihao  Han Yifeng  Han Feng  Li Xin  
【摘要】:As a post-translational modification, protein S-nitrosylation has been increasingly recognized as an important signaling process in vascular physiology and pathology,[1] and its in situ detection would provide valuable information to interrogate the molecular mechanisms underpinning related-diseases. Motivated by the dearth of chemical tools capable of directly imaging protein S-nitrosylation in biological contexts, we have developed a fluorogenic probe based on the phosphine-RSNO chemistry. The probe is weakly emissive alone and becomes highly fluorescent only after undergoing reaction with S-nitrosothiols in live cellular environments.[2] It has been successfully applied to image the dynamic changes of protein S-nitrosylation in live endothelial cells. Its specificity in complex biological systems has been further verified by imaging a known target of S-nitrosylation, glyceraldehyde-3-phosphate dehydrogenase(GAPDH), in live cells.

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