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A Novel Approach for Discovering Trace Sulfated N-Glycans on IgGs as Biomarkers for Rheumatoid Arthritis

Jing-Rong Wang  Wei-Na Gao  Shibo Jiang  Yong Liang  Lee-Fong Yau  Hao Huang  Ju Liu  Min Jiang  Qiong Meng  Tian-Tian Tong  Liang Liu  Zhi-Hong Jiang  
【摘要】:正The biological functions of N-linked glycans on immunoglobulin G(IgG)are closely associated with pathogenesis and therapeutic actions;however,certain pivotal low-abundance species,such as acidic N-glycans on IgGs,are difficult to detect.Here,we report a novel glycomic approach based on a specialized microfluidic chip that enables the identification of numerous N-glycans on human serum IgGs.Notably,modified N-glycans,including 20 sulfated,

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