A Novel Approach for Discovering Trace Sulfated N-Glycans on IgGs as Biomarkers for Rheumatoid Arthritis

【摘要】：正The biological functions of N-linked glycans on immunoglobulin G(IgG)are closely associated with pathogenesis and therapeutic actions;however,certain pivotal low-abundance species,such as acidic N-glycans on IgGs,are difficult to detect.Here,we report a novel glycomic approach based on a specialized microfluidic chip that enables the identification of numerous N-glycans on human serum IgGs.Notably,modified N-glycans,including 20 sulfated,