Measuring the Stoichiometry of Functional PspA Complexes in Living Bacterial Cells by Single Molecule Photobleaching
【摘要】:正Proteins often function as multimeric complexes in cellular processes such as signal transduction and ion transport.Many well-established techniques exist to determine the structure and composition of protein complexes in their isolated forms in vitro,yet it is challenging to measure the stoichiometry of functional complexes in living cells.Here we
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