收藏 | 手机打开

BmP02 Atypically Delays Kv4.2 Inactivation:Implication for a Unique Interaction between Scorpion Toxin and Potassium Channel

Bin Wu  Yan Zhu  Jian Shi  Jie Tao  Yonghua Ji  
【摘要】:BmP02,a short-chain peptide with 28 residues from the venom of Chinese scorpion Buthus martensi Karsch,has been reported to inhibit the transient outward potassium currents(I_(to)) in rat ventricular muscle cells.However,it remains unclear whether BmP02 modulates the Kv4.2 channel,one of the main contributors to I_(to).The present study investigated the effects of BmP02 on Kv4.2 kinetics and its underlying molecular mechanism.The electrophysiological recordings showed that the inactivation of Kv4.2 expressed in HEK293 T cells was significantly delayed by BmP02 in a doseresponse manner with EC_(50) of~850 nM while the peak current,activation and voltage-dependent inactivation of Kv4.2 were not affected.Meanwhile,the recovery from inactivation of Kv4.2 was accelerated and the deactivation was slowed after the application of BmP02.The site-directed mutagenesis combined with computational modelling identified that K347 and K353,located in the turret motif of the Kv4.2,and E4/E5,D20/D21 in BmP02 are key residues to interact with BmP02 through electrostatic force.These findings not only reveal a novel interaction between Kv4.2 channel and its peptidyl modulator,but also provide valuable information for design of highly-selective Kv4.2 modulators.

中国期刊全文数据库 前19条
1 ;Pharmacological kinetics of BmK AS, a sodium channel site 4-specific modulator on Na_v1.3[J];Neuroscience Bulletin;2012年03期
3 BYUNG-MIN YU;JEONG-MIN LEE;CHRISTIAN MAI;STEFAN LISCHKE;LARS ZIMMERMANN;WOO-YOUNG CHOI;;Single-chip Si optical single-sideband modulator[J];Photonics Research;2018年01期
4 唐平华;陶月;毛宇亮;吴熳;黄宗玉;梁胜男;陈新行;祁祥;黄斌;刘军;赵楚军;;Graphene/MoS_2 heterostructure: a robust mid-infrared optical modulator for Er~(3+)-doped ZBLAN fiber laser[J];Chinese Optics Letters;2018年02期
5 王奕方;吴侃;陈建平;;All-optical modulator based on MoS_2-PVA thin film[J];Chinese Optics Letters;2018年02期
6 谢一言;杨杨;韩璐;岳庆炀;国承山;;Generation of arbitrary vector beams based on a single spatial light modulator and a thin-film polarization splitting cubic[J];Chinese Optics Letters;2016年12期
7 王建帅;裴丽;翁思俊;吴良英;宁提纲;李晶;;All-fiber electro-optic modulator based on D-shaped twin-core fiber[J];Chinese Optics Letters;2016年10期
8 Yanyang Zhou;Linjie Zhou;Haike Zhu;Chiyan Wong;Yida Wen;Lei Liu;Xinwan Li;Jianping Chen;;Modeling and optimization of a single-drive push–pull silicon Mach–Zehnder modulator[J];Photonics Research;2016年04期
9 王敏娟;周林杰;朱海柯;周砚扬;钟一鸣;陈建平;;Low-loss high-extinction-ratio single-drive push-pull silicon Michelson interferometric modulator[J];Chinese Optics Letters;2017年04期
10 高迪;李培丽;邵茜;陈静远;;Generation of ultra-wide and flat optical frequency comb based on electro-absorption modulator and frequency modulator[J];Optoelectronics Letters;2016年02期
11 张彬;刘诗雨;唐先柱;陆建钢;;Adaptive modulation system for liquid crystal phase modulator[J];Chinese Optics Letters;2016年09期
12 武海军;李斌;张华斌;李正平;曾隆月;;A single die 1.2 V 55 to 95 dB DR delta sigma ADC with configurable modulator and OSR[J];Journal of Semiconductors;2014年03期
13 陈铭易;周立国;边程浩;颜峻;石寅;;A 2.52-mW continuous-time Σ△ modulator with 72 dB dynamic range for FM radio[J];Journal of Semiconductors;2014年10期
14 袁宇丹;李立;常虹;郭亚炜;程旭;曾晓洋;;A 100-MHz bandpass sigma-delta modulator with a 75-dB dynamic range for IF receivers[J];半导体学报;2011年02期
15 李金凤;唐祯安;;An A/D interface based on ∑△ modulator for thermal vacuum sensor ASICs[J];半导体学报;2010年07期
16 葛彬杰;王新安;张兴;冯晓星;汪清勤;;Sigma-delta modulator modeling analysis and design[J];半导体学报;2010年09期
17 Amal K Ghosh;Moumita Raul;;Trinary registers and counters using savart plate and spatial light modulator for optical computation in multivalued logic[J];Optoelectronics Letters;2009年03期
18 朱颖佳;刘力源;李冬梅;;An 11 mW 79 dB DR △Σ modulator for ADSL applications[J];半导体学报;2009年10期
19 ;New modulator for the optical signal in a fiber[J];Optoelectronics Letters;2006年06期
中国重要会议论文全文数据库 前10条
1 Bin Wu;Yan Zhu;Jian Shi;Jie Tao;Yonghua Ji;;BmP02 Atypically Delays Kv4.2 Inactivation:Implication for a Unique Interaction between Scorpion Toxin and Potassium Channel[A];第十四届生物毒素毒理学术大会暨第一届生物毒素——从生存适应到转化医学专题学术会议会刊[C];2019年
2 Lifang Sun;Xueji Wu;Yu Peng;Yufen Zhao;Donghai Lin;;Solution structural analysis of the single-domain parvulin TbPin1[A];生命的分子机器及其调控网络——2012年全国生物化学与分子生物学学术大会摘要集[C];2012年
3 ;Three-Phase Four-Leg Active Power Filter Based on Nonlinear Optimal Predictive Control[A];第二十七届中国控制会议论文集[C];2008年
4 井媛媛;Xiaochao Wang;Wei Fan;Zhi Qiao;Xin Chen;;1053-nm all-fiber multi-pass phase modulator for chirped pulsed amplification[A];激光聚变能源检测与驱动技术研讨会摘要集[C];2015年
5 黄志敏;;ASD:a comprehensive database of allosteric proteins and modulators[A];第十一届全国计算(机)化学学术会议论文摘要集[C];2011年
6 Di Fan;Ruirui Wang;Jun Chang;;A new method for testing the asphere surface with a liquid crystal spatial light modulator[A];第十四届全国光学测试学术讨论会论文(摘要集)[C];2012年
7 ;A Sixth-order MASH Algorithm used in ∑-Δ ADC[A];四川省电子学会半导体与集成技术专委会2006年度学术年会论文集[C];2006年
8 ;Rat pain-related responses induced by intraplantar injection of BmK I,a sodium channel modulator from scorpion Buthus Martensi Karsch[A];第八届中国生物毒素学术研讨会论文摘要集[C];2007年
9 Kang-Dong Liu;Fang Bai;Jun-Sheng Zhu;Jia-Wei Wang;Wei-Dong Zhang;Zi-Gang Dong;Hong-Lin Li;;Veratramine, a natural modulator of AP-1-dependent gene transcription via direct binding the programmable DNA[A];中国化学会第十届全国天然有机化学学术会议论文集——第三分会场:天然产物合成生物学、化学生物学及新技术[C];2014年
中国博士学位论文全文数据库 前1条
1 Ahmed Hosni Ali Mohamed Elghandour;[D];长春理工大学;2013年
中国硕士学位论文全文数据库 前1条
1 Mueed Ur Rahman;[D];上海交通大学;2017年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978