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Spinal 5-HT3AR contributes to BmK I-induced inflammatory pain in rats

Jin Fu  Yu-Lu Jiao  Yonghua Ji  
【摘要】:Subcutaneous injection of BmK I could be adopted to well establish a novel pain model.Moreover,5-hydroxytryptamine(serotonin,5-HT) receptor is involved in regulating animal pain-related behaviors.However,the underlying mechanism of 5-HT3 R on BmK I-induced pain remains unclear.Animal behavioral testing,RT-PCR and Western blotting were used to yield the following results:first,intraplantar(i.p1.) injection of BmK I(10 μg) induced elevated mRNA and protein levels of 5-HT3 AR in bilateral L4-L5 spinal cord;Second,intrathecal(i.t.) injection of ondansetron(a specific antagonist of 5-HT3AR) reduced spontaneous pain responses,attenuated unilateral thermal and bilateral mechanical hypersensitivity elicited by BmK I;Microglia could be activated by BmK I(i.pl.) in both sides of L4-L5 spinal cord,and this effect was reversed by intrathecal pre-treatment with 5-HT3 AR antagonist.Meanwhile,the 5-HT3 AR in L4-L5 spinal cord was almost co-localized with NeuN(a marker of nerve cell),but not co-expressed with Iba-1(a marker of microglia).Finally,the expression level of CX3CL1 and CX3CR1 was reduced by intrathecal pre-treatment with ondansetron.Our results indicate that both 5-HT3 AR signaling pathway and microglia are activated in the process of induction and maintenance of BmK I-induced pain nociception.Meanwhile,the results suggest that the neuronal 5-HT3 AR may communicate with microglia indirectly via CX3CL1 which is involved in regulating the BmK I-induced hyperalgesia and sensitization.~1

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1 李明杰;陈志敏;郑建仲;;Observation by Fluorescent Histochemical Technique and Estimation of Catecholamine Content in Cerebral Cortex following Experimental Cerebral Infarction[J];Journal of Medical Colleges of PLA;1988年01期
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