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Dynamic expression of VGSCs subtypes Nav1.1,Nav1.2,Nav1.3 and Nav1.6 triggered by BmK I in rat hippocampus

Hao Wu  Hongyan Zhu  Yonghua Ji  
【摘要】:BmK I(an-like toxin purified from the venom of Eastern Asian scorpion Buthus martensi Karsch) has been identified to a site-3 specific modulator of VGSCs with capacity ofinducing convulsive motor activities by intrahippocampal injection.Here,BmK I was unilaterally injected to the hippocampus in rats to evoke convulsive motor activities,then the expression of VGSCs subtypes Nav1.1,Nav1.2,Nav1.3 and Nav1.6 were detected at 1 day,7 days,21 days and 63 days after BmK I injection.Immunostaining revealed that the expression of Nav1.1,Nav1.2,Nav1.3and Nav1.6 was robustly up-regulated in the ipsilateral hippocampal CA1 and CA3 subregions at 7days,21 days after Bmk I injection compared with that of the contralateral side and control hippocampus,reaching the peak at 21 days after Bmk I injection.Surprisingly,Nav1.1,Nav1.2,Nav1.3 and Nav1.6 immunoreactivities cannot be detected in the ipsilateral hippocampus at 63 days after BmK I injection.Furthermore,a large number of Nav1.1,Nav1.2,Nav1.3 and Nav1.6immunoreactivities were found to be localized in reactive astrocytes(GFAP-positive) at 7 days,21 days after BmK I injection.Quantitative real-time PCR also address the dramatic up-regulation of Nav1.1,Nav1.2,Nav1.3 and Nav1.6 expression at 21 days after BmK I injection with that of the contralateral side and control hippocampus.Theresults suggest that BmK I,a specific VGSCs modulator,not only can induce the seizure-like activities but also affect the expression of brain subtype VGSCs with time-dependent pattern.

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