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Proteomics identification of heat shock protein 27 and cyclophilin A participate in the pathogenesis of COPD

【摘要】:正Background and objective:COPD is a public health problem worldwide with complex pathogenesis, and has not been well understood.The current study aims to acquire new clues for the pathogenesis of COPD by use of proteomics technology.Methods:Lung tissue specimens were harvested from never-smokers,non-COPD smokers and COPD smokers,and the proteins differentially expressed between the 3 group subjects were revealed by two dimensional electrophoresis and sequence identified by matrix assisted laser desorption/ionization time of flight mass spectrometry analysis combined with bioinformation technology.The interested differentially expressed proteins identified by proteomics technology were validated by immunohistochemistory and Western-blotting.Results: Forty seven protein spots were revealed to be differentially expressed between groups with intensity variation more than 1.5 times from two dimensional electrophoresis maps,and 24 proteins were identified through mass spectrometry analysis combined with database enquiry.Differentially expressed proteins were functionally mainly involved in basic metabolism,oxidoreduction,coagulation/fibrinolysis,protein degradation,signal transduction, inflammation,cell growth/differentiation.About the 24 identified proteins,22 proteins were up-regulated in the lung of smokers compared with never-smokers.The results of proteomics,immunohistochemical staining and Western-blotting accordant demonstrated that the expression of heat shock protein 27 and Cyclophilin A in lung was increased in non-COPD smokers and further up-regulated in COPD smokers when compared with never-smokers. Conclusion:Heat shock protein 27 and Cyclophilin A participate in the pathogenesis of COPD,and their expression in lung is induced by smoking.

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