Apelin-13 promotes monocyte adhesion to human umbilical vein endothelial cell mediated by phosphatidylinositol 3-kinase signaling

【摘要】：正Aim:To observe the effects of apelin-13 induced monocytes adhesion to human umbilical vein endothelial cells(HUVECs) and confirm the function of phosphatidylinositol 3-kinase(PI3K) in this process.Methods: Human umbilical vein endothelial cell line ECV304 was cultured in DMEM and the monocyte cell line THP-1 was cultured in 1640 medium.MPO assay was used to identify effects of monocytes adhesion to HUVECs.Expressions of vascular cell adhesion molecule(VCAM)-1,phospho-PI3K and PI3K were detected by Western Blotting.Results: Myeloperoxidase(MPO) assay results showed that apelin-13 induced monocyte adhesion to HUVECs in concentration -and time-dependent manner,which reached their peaks at 1μmol/L[adhesion rate=(74.2±0.23)%]and 12 h [adhesion rate=(67.9±2.40)%],respectively.And the PI3K inhibitor LY294002 inhibited the monocytes adhesion to HUVECs induced by apelin-13.Western Blotting analysis suggested that apelin-13 significantly promoted the expressions of VCAM-1 in concentration- and time-dependent manner,which reached the peak level at 1μmol/L and 12 h respectively.Furthermore,Apelin-13 promoted the expression of phospho-PI3K in concentration- and time-dependent manner,which reached the peak level at 1μmol/L and 30 min respectively.LY294002 inhibited the expression of phospho-PI3K in HUVECs induced by apelin-13.However,apelin-13 + LY294002 had no effect on PI3K expression.Conclusion:Apelin-13 promoted monocytes adhesion to HUVECs through VCAM-1 mediated by PI3K signal cascades.