收藏本站
收藏 | 手机打开
二维码
手机客户端打开本文

Frequency representation and tuning of the inferior collicular neurons of mice:intracellular recording in vivo

【摘要】:正Sound frequency is one of the three basic characteristic of the sound,each central auditory neuron can respond to sound within some range of frequency,represent,and code these sound frequencies by different pattern of membrane potential.Because there are some difficulties in using intracellular recording in vivo,previous studies on frequency representation and tuning of sound in central auditory neurons were basically to use electrophysiological method of extracellular recording in vivo.In this study,under free field stimulation condition,we used the method of intracellular recording in vivo and investigated frequency representation and tuning of inferior collicular(IC) neurons in light anesthetized mice(Mus musculus,Km).80 sound sensitive IC neurons were obtained by intracellular recording and frequency representation and tuning of 39 among these neurons were analyzed.The ranges(mean±standard deviation) of recording depth(urn),best frequency(kHz),frequency bandwidth(kHz),and response latency(ms) of 39 neurons were 345-1871(963±352),5-33(17.0±8.6),7-47(30.3±11.7),and 3.5-19.2(11.2±3.8),respectively.According to responses and representation of neurons to different sound frequencies,they could be classified into four types, i.e.(1) the neurons(n=9) showed sound frequency representation and tuning property by inhibitory postsynaptic potential (IPSP) and duration of inhibitory action;(2) the neurons(n=7) showed sound frequency representation and tuning property by excitatory postsynaptic potential(EPSP);(3) the neurons(n=18) showed sound frequency representation and tuning property by action potential(AP);and(4) the neurons(n=5) showed sound frequency representation and tuning property by different ways of membrane potentials.On the other hand,the neurons had the shortest latency to best frequency sound stimulus and their latencies also showed some tuning properties,latency changed with frequency changing.By comparison of latencies of first IPSP,EPSP and AP,the results showed latency(8.8±3.4) ms of IPSPlatency(9.3±4.0) ms of EPSPlatency(12.8±3.2) ms of AP,and there was a statistical significance(P0.05) among changes in the latencies.The shortest latency of IPSP showed that inhibitory input evoked by sound stimulus was earlier arriving to recorded neuron than excitatory input.Exception for rise times of IPSP and EPSP,the amplitudes, duration,and decay times of IPSP and EPSP varied with frequency changing.Mean duration(285.3±187.5) ms, rise time(22.1±13.1) ms,and decay time(263.3±182.6) ms of IPSP evoked by BF sound stimulus were longer than mean duration(37.6±68.1) ms,rise time(5.0±6.5) ms,and decay time(32.6±62.0) ms of EPSP(P0.05).But there was no statistical significance(P0.05) between mean amplitudes of IPSP(7.0±3.5) mV and EPSP(5.3±2.1) mV.These results from intracellular recording demonstrated that three ways,AP,EPSP and EPSP,could be used in frequency representation and tuning of IC neurons,but their temporal properties were different.The longer duration of IPSP suggested that IC neurons needed longer recovery time from hyper-polarization to resting membrane potential(RP) than from EPSP to RP.

知网文化
【相似文献】
中国重要会议论文全文数据库 前2条
1 ;Frequency representation and tuning of the inferior collicular neurons of mice:intracellular recording in vivo[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
2 ;Influence of binocular motion on disparity tuning in cat area 18[A];Proceedings of the 8th Biennial Conference of the Chinese Society for Neuroscience[C];2009年
中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978