收藏本站
收藏 | 手机打开
二维码
手机客户端打开本文

Histamine promotes rat motor performances through activation of H_2 receptors in cerebellar fastigial nucleus

【摘要】:正The cerebellar fastigial nucleus(FN),together with the interpositus nucleus(IN),constitutes the two final output nuclei of the spinocerebellum and holds an important role in motor control and body balance.Previous studies have revealed a direct histaminergic projection from the hypothalamus to the cerebellar nuclei and a postsynaptic excitatory effect of histamine on the cerebellar IN.However,the effect of histamine and physiological significance of hypothalamic histaminergic projection on the cerebellar FN has been still unknown.In this study,using rat brain slice preparations and extracellular recordings,we found that histamine elicited the cerebellar FN neurons a concentration-dependent excitatory response(65/77,84.4%).The low-Ca~(2+)/high-Mg~(2+) medium did not block the histamine-induced excitation(n = 10),indicated a direct postsynaptic action of the amine.The excitation of histamine on FN neurons was not blocked by selective histamine H_1 receptor antagonist triprolidine(n = 20) or chlorpheniramine(n = 10),but was effectively suppressed by ranitidine(n = 15),a selective histamine H_2 receptor antagonist.On the other hand,selective histamine H_2 receptor agonist dimaprit(n = 30) instead of histamine H_1 receptor agonist 2-pyridylethylamine(n = 20) mimicked the excitatory effect of histamine on FN neurons.The dimaprit-induced FN neuronal excitation was effectively antagonized by ranitidine(n = 13) but not triprolidine(n = 15).These results demonstrate that histamine excites cerebellar FN cells via the histamine H_2 receptor mechanism. Furthermore,histaminergic agents were bilaterally microinjected into the cerebellar FN of adult male rats in vivo for observation of their effects on motor behavior.Although histamine did not influence general movements in an open field,it remarkably improved motor performances in accelerating rota-rod,balance beam,and suspension tests. Ranitidine considerably declined those motor performances,but triprolidine showed no effect.Dimaprit rather than 2-pyridylethylamine mimicked the facilitatory effect of histamine on the movements.Moreover,histamine reversed the inhibitory effects of ranitidine on motor performances.These results demonstrate that histamine enhances rat motor balance and coordination through modulation of cerebellar FN neurons by activation of histamine H_2 receptors, and suggest that the hypothalamocerebellar histaminergic projections originating from the hypothalamus may modulate the cerebellar final output nuclei and subsequently influence the cerebellar function of motor control.Considering the hypothalamus is a high center for regulating visceral activities,we speculate that during ongoing movements, the hypothalamocerebellar histaminergic pathway may help to generate an integrated and coordinated somatic-visceral response for adaption of the changes of internal and external environments.

知网文化
【相似文献】
中国重要会议论文全文数据库 前10条
1 ;Histamine promotes rat motor performances through activation of H_2 receptors in cerebellar fastigial nucleus[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
2 ;Histamine excites rat spinalα-motoneurons and the underlying mechanisms[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
3 ;The role of histamine on adipogenesis and energy metabolism in adipocytes[A];湖南省生理科学会2006年度学术年会论文摘要汇编[C];2007年
4 ;Store-operated Ca2+ entry participated in Th2 response induced by histamine in human monocyte-derived dendritic cells[A];中华医学会呼吸病学年会——2011(第十二次全国呼吸病学学术会议)论文汇编[C];2011年
5 Shalakhmetova T.M.;Askarova S.N.;Umbayev B.A.;and Tsoy A.K.;;EXPRESSION OF P-SELECTIN AND ACTIN POLYMERIZATION OF CEREBRAL ENDOTHELIAL CELLS UNDER EXPOSURE OF Aβ AND THERAPEUTIC PROPERTIES OF LOVASTATIN[A];中国的遗传学研究——遗传学进步推动中国西部经济与社会发展——2011年中国遗传学会大会论文摘要汇编[C];2011年
6 黄卫;黄熙;邢之华;邱新建;王杨;刘卫平;任平;唐涛;张春虎;刘昭前;周宏灏;;Meranzin hydrate induces similar effect to Fructus Aurantii on intestinal motility through activation of H1 histamine receptors[A];第六届全国中西医结合基础理论研究学术研讨会暨第二届湖南省中西医结合学会肝病专业学术年会论文集[C];2010年
7 ;Histamine excites subthalamic nucleus neurons and improves motor behavior in normal and Parkinsonian rats[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
8 ;Histamine modulates activity and firing pattern of the subthalamic nucleus neurons and improves motor behaviors in a rat model of Parkinson's disease[A];中国生理学会第九届全国青年生理学工作者学术会议论文摘要[C];2011年
9 高玲;费素娟;张建福;邢宏彬;乔伟丽;;刺激小脑顶核对大鼠应激性胃黏膜损伤的神经调控[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
10 田学隆;刘虔铖;;电刺激小脑顶核对疾病的预防和治疗作用[A];中国仪器仪表学会医疗仪器分会第四次全国会员代表大会暨2009年学术年会论文集[C];2009年
中国博士学位论文全文数据库 前4条
1 谢铮;黄芩汤提取物加减滴鼻液对实验性变应性鼻炎豚鼠模型的影响[D];北京中医药大学;2010年
2 杨瑞瑞;诱发眼震电图动态监测小脑出血的应用基础研究[D];重庆医科大学;2010年
3 何功浩;交感神经组胺的突触效应及其在急性缺血性心律失常中的病理生理功能的研究[D];第四军医大学;2010年
4 陆健花;小脑间位核调节免疫功能的作用途径研究[D];苏州大学;2012年
中国硕士学位论文全文数据库 前9条
1 杨健;水产品中组胺产生的微生物机制[D];宁波大学;2012年
2 杨芬妍;吲哚(吡啶)烷胺类衍生物的设计合成及其H_3受体拮抗/激动活性研究[D];浙江大学;2011年
3 汪晓玲;TERT在预电刺激小脑顶核后脑缺血再灌注保护机制中的作用[D];广西医科大学;2010年
4 陈瑛瑛;交感神经源性组胺对兔耳血管舒缩功能的影响[D];第四军医大学;2010年
5 寿雯婷;组氨酸脱羧酶基因敲除小鼠痛觉感受及初级感觉神经元中Na_v1.8改变的研究[D];浙江大学;2011年
6 王锦;电刺激小脑顶核对容积脉搏波参数影响的研究[D];重庆大学;2010年
7 杨乙;电刺激小脑顶核诱导脑缺血再灌注大鼠TERT的表达及其对线粒体凋亡途径的影响[D];广西医科大学;2011年
8 何旭东;乌灵菌粉对戊四唑点燃癫痫大鼠脑内组胺及pCREB表达的影响[D];浙江大学;2010年
9 欧书;电刺激小脑顶核对偏头痛预防治疗的临床研究[D];重庆医科大学;2012年
中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978