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Inhibition controls sensory information processing in cerebellar cortex in mice

【摘要】:正Objective:Although the sensory stimulus precisely evokes response in Purkinje cells(PC),the cellular mechanisms of sensory information processing in cerebellar cortex are unclear.In the present study,we aimed to study the cerebellar PCs in response to natural stimulus on ipsilateral whisker pad by in vivo whole-cell recording methods.Methods:Adult(6-8 weeks old) ICR mice were anesthetized with urethane.After a watertight chamber was created around the hemisphere,a craniotomy was drilled to expose the cerebellar surface in correspondence to CrusⅡ.Tactile stimulus on ipsilateral whisker pad was performed by air-puff through a stainless tube connected with a pressurized injection system.Membrane potentials and/or currents were monitored with an Axopatch-1D amplifier,acquired through a Digidata 1200 series analog-to-digital interface on a personal computer using Clampex 8.1 software.Differences between the mean values recorded under control and test conditions were evaluated by Student's paired t-test using SPSS software.Results:We found that air-puff stimulus on trigeminal afferents evoked neither complex spike nor simple spike,but indeed elicited a strong GABA_A receptor-mediated inhibition of PCs in cerebellar cortex folium CrusⅡin urethane anesthetized mice.Field potential recordings from both molecular layer and PC layer showed that air-puff stimulus evoked a sequence of parallel fiber volley followed by a GABA_A receptor-mediated inhibition,which was completely blocked by AMPA receptor antagonist.Cell-attached recordings from PCs showed that air-puff stimulus evoked a pause of spike firing,GABA_A receptor antagonist abolished the pause,revealed the evoked firing of these PCs in response to the stimulus.Under current-clamp,air-puff stimulus did not evoke complex spike or simple spike in PCs,but induced strong inhibitory postsynaptic potential (IPSP) in both PCs somata and dendrites.In the presence of GABA_A receptor antagonist,air-puff stimulus could not evoke complex spike,but fired simple spike in PCs.Under voltage-clamp,air-puff stimulus evoked a sequence of tiny transient inward current followed by strong outward current in both PC somata and dendrites.Blockade of inhibition revealed strong excitatory postsynaptic currents in PC somata and a temporal summation of parallel fiber excitatory inputs onto PC dendrites.Conclusion:These results indicated that natural stimulus on trigeminal afferents neither evoked complex spikes through climbing fibers,nor fired simple spikes via parallel fibers,but induced strong inhibition in PCs,suggesting that the inhibition controls sensory information processing in cerebellar cortex folium CrusⅡin vivo in mice.

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