【摘要】：正Objective To explore the proliferation and differentiation-inducing effects of aminosteroid H42648 on K562 leukemia cells.Methods The effects of H42648 on K562 cell proliferation were studied by cell count,colony count and MTT assay,the differentaition by morphology, benzidine stain and flow cytometry.Results The growth of K562 cells was inhibited after treatment with H42648 for 5 days.The inhibition rates for K562 cells in MTT assay were(26.90±3.18)%and(43.26±2.54)%respectively after treatment by 10~(-8)mol/L and 10~(-6)mol/L H42648.The inhibition for K562 cells in colony culture assay were(22.21±9.20)%and(48.71±8.24)%at the dose of 10~(-8)mol/L and 10~(-6)mol/L H42648 respectively.H42648 also induced K562 cells toward erythroid differetaition.It was verified by bezindine stain in that the OD value at the dose of 10~(-8)mol/L H42648 was increased by(153±17.65)% compared with the control,and at the dose of 10~(-6)mol/L H42648 the OD value was increased by(250±4.60)%compare with the control.The morphology showed the differetiation tendency after treatment of K562 cells.Data from flow cytometry showed that after treatment with 10~(-6)mol/L H42648 for 4 days,the percentage of positive CD71 expression in differentiated K562 cells was 89.91%.Conclusion H42648 can inhibit the proliferation of K562 cell lines and induce toward erythriod differetiation.H42648 may become a new lead for the differentiation-inducing therapy of leukemia.