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Genome-wide analyses of chromatin interactions after the loss of Pol Ⅰ,Pol Ⅱ and Pol Ⅲ

Yongpeng Jiang  Jie Huang  Kehuan Lun  Boyuan Li  Haonan Zheng  Yuanjun Li  Rong Zhou  Wenjia Duan  Chenlu Wang  Yuanqing Feng  Hong Yao  Cheng Li  Xiong Ji  
【摘要】:Background:The relationship between transcription and the 3 D chromatin structure is debated.Multiple studies have shown that transcription affects global Cohesin binding and 3 D genome structures.However,several other studies have indicated that inhibited transcription does not alter chromatin conformations.Results:We provide the most comprehensive evidence to date to demonstrate that transcription plays a relatively modest role in organizing the local,small-scale chromatin structures in mammalian cells.We show degraded Pol Ⅰ,Pol Ⅱ and Pol Ⅲ proteins in mESCs cause few or no changes in large-scale 3 D chromatin structures,selected RNA polymerases with a high abundance of binding sites or active promoterassociated interactions appear to be relatively more affected after the degradation,transcription inhibition alters local,small loop domains,as indicated by high-resolution chromatin interaction maps,and loops with bound Pol Ⅱ but without Cohesin or CTCF are identified and found to be largely unchanged after transcription inhibition.Interestingly,Pol Ⅱ depletion for a longer time significantly affects the chromatin accessibility and Cohesin occupancy,suggesting that RNA polymerases are capable of affecting the 3 D genome indirectly.These direct and indirect effects explain the previous inconsistent findings on the influence of transcription inhibition on the 3 D genome.Conclusions:We conclude that Pol Ⅰ,Pol Ⅱ,and Pol Ⅲ loss alters local,small-scale chromatin interactions in mammalian cells,suggesting that the 3 D chromatin structures are pre-established and relatively stable.

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中国重要会议论文全文数据库 前1条
1 Yongpeng Jiang;Jie Huang;Kehuan Lun;Boyuan Li;Haonan Zheng;Yuanjun Li;Rong Zhou;Wenjia Duan;Chenlu Wang;Yuanqing Feng;Hong Yao;Cheng Li;Xiong Ji;;Genome-wide analyses of chromatin interactions after the loss of Pol Ⅰ,Pol Ⅱ and Pol Ⅲ[A];中国生物化学与分子生物学会2020全国学术在线会议摘要集[C];2020年
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