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Members of miR-169 family are induced by high salinity and transient inhibit NF-YA transcriptional factors

【摘要】:正MicroRNAs(miRNAs)are endogenously expressed small RNAs with length of~22nt. MicroRNAs silence its target genes at post-transcriptional level.In plants,microRNAs play various developmental and physiological roles by cleavaging mRNAs dominantly.Drought and high salinity are the most severe environment abiotic stresses and cause the crop loss all over the word.In this study,we identified miR-169g,miP,-169n(o)as high salinity responsive microRNAs in rice.MiR-169n and miR169o were in a microRNA cluster with a distance of 3707bp.The high degree of conservation and close phylogenic distance of the pre-miR-169n and pre-miR-169o indicated that they derived from a tandem duplication evolutional event which occurred very recently.The existence of an ABRE cis-acting element at the upstream region of miR-169n(o) suggested that miR-169n(o)may be regulated by ABA.In our previous study,we found that the osmotic stress resulted by drought induced miR-169g through DRE element.Here,we revealed that the osmotic and ionic toxicity stress resulted by high salinity induced miR-169g and miR-169n(o)through both DRE and ABRE element.Our data showed that the both overlap and distinct responses of miR-169 family to drought and salt stress.We also showed that these miR-169 members selectively cleaved some of NF-YA genes which are CCAAT-box binding transcription factors and participate in transcriptional regulation of large number genes.Finally, we found one or more ath-miR-169 member also induced by high salinity.It indicated that the salt-induction of some miR-169 members was a general property in plants.

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