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Lactobacillus Rhamnosus GG Attenuates Tenofovir Disoproxil Fumarate-induced Bone Loss in Male Mice via Gut Microbiota-dependent Anti-inflammation

Hao Liu  Ranli Gu  Wei Li  Wen Zhou  Zhe Cong  Jing Xue  Yunsong Liu  Qiang Wei  Yongsheng Zhou  
【摘要】:Background:Although anti-retroviral agent widely triggered bone loss in HIV patients,tenofovir disoproxil fumarate(TDF) indeed induces severer bone damage like osteoporosis.However,the mechanisms is obscure.However,probiotic supplements may be effective against osteoporosis.Methods:C57 BL6/J mice were administered Lactobacillus rhamnosus GG(LGG)+TDF,TDF,and zoledronate+TDF,respectively.Next,bone morphometry and biomechanics was evaluated by micro-CT,bone slicing,and flexural tests.The lymphocyte,proinflammatory cytokines,and intestinal permeability were detected by ELISA,qPCR,and flow cytometry.The gut microbiota composition and metabolomics were analyzed by 16 S r DNA pyrosequencing and UPLC-Q-TOF/MS.Results:LGG administered orally marked increases in trabecular bone microarchitecture,cortical bone volume,and biomechanical properties in LGG+TDF group compared with that in the TDF only group.Moreover,LGG treatment increased intestinal barrier integrity,expanded regulatory T cells,decreased Th17 cells,and downregulated osteoclastogenesis-related cytokines in the bone marrow,spleen,and gut.Furthermore,LGG reconstructed the gut microbiota and changed the metabolites composition,especially lysophosphatidycholines levels.However,the amount of N-Acetyl-leukotriene E4 was the highest in the TDF only group.Conclusion:LGG reconstructed the community structure of the gut microbiota,promoted the expression of lysophosphatidycholines and improved intestinal integrity to suppress TDF-induced inflammatory response,which resulted in attenuation of TDF-induced bone loss in mice.It may be safe and effective strategy of using probiotics to prevent and/or treat TDF-induced osteoporosis.

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3 Zhan-Yu Yang;Jiang-Dong Ni;Ze Long;Le-Tian Kuang;Shi-Bin Tao;;Unusual presentation of congenital radioulnar synostosis with osteoporosis,fragility fracture and nonunion:A case report and review of literature[J];World Journal of Clinical Cases;2020年08期
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5 Andreia Machado Silva;Sara Reis Moura;José Henrique Teixeira;Mário Adolfo Barbosa;Susana Gomes Santos;Maria Inês Almeida;;Long noncoding RNAs:a missing link in osteoporosis[J];Bone Research;2019年01期
6 Candice L Downey;Adam Young;Emily F Burton;Simon M Graham;Robert J Macfarlane;Eva-Maria Tsapakis;Eleftherios Tsiridis;;Dementia and osteoporosis in a geriatric population: Is there a common link?[J];World Journal of Orthopedics;2017年05期
7 Farangis Fatehi;Majid Mollahosseini;Gholamhossein Hassanshahi;Soudeh Khanamani Falahati-pour;Hossein Khorramdelazad;Zahra Ahmadi;Mojgan Noroozi Karimabad;Habib Farahmand;;CC chemokines CCL2, CCL3,CCL4 and CCL5 are elevated in osteoporosis patients[J];The Journal of Biomedical Research;2017年05期
8 何维祯;朱昀浩;封婷;袁杰;程茜;徐冠;王学鼎;;Comparison study of photoacoustic and ultrasound spectrum analysis in osteoporosis detection[J];Chinese Optics Letters;2017年11期
9 Yasser El Miedany;Maha El Gaafary;Ahmed El Yassaki;Sally Youssef;Annie Nasr;Ihab Ahmed;;Monitoring osteoporosis therapy:Can FRAX help assessing success or failure in achieving treatment goals?[J];World Journal of Rheumatology;2014年02期
10 Andrea Montagnani;;Bone anabolics in osteoporosis: Actuality and perspectives[J];World Journal of Orthopedics;2014年03期
11 Mohammad Reza Etemadifar;Sayed-Mohammadamin Nourian;Mahboobe Fereidan-Esfahani;Hamidreza Shemshaki;Mohsen Nourbakhsh;Abolghasem Zarezadeh;;Relationship of knowledge about osteoporosis with education level and life habits[J];World Journal of Orthopedics;2013年03期
12 Carla Andrade Lima;Andre Castro Lyra;Raquel Rocha;Genoile Oliveira Santana;;Risk factors for osteoporosis in inflammatory bowel disease patients[J];World Journal of Gastrointestinal Pathophysiology;2015年04期
13 YANG Xian-lin;HE Ming;JIANG Shan;SHEN Hong-tao;MI Sheng-quan;ZHAO Qing-zhang;ZHANG Yu-xuan;WANG Fang-fang;WU Shao-yong;;Application Research of Calcium Absorption Rate of Osteoporosis Rats Using ~(41)Ca-AMS Technology[J];Annual Report of China Institute of Atomic Energy;2016年00期
14 Qiang Wang;Bing Zhao;Chao Li;Jie-Sheng Rong;Shu-Qing Tao;Tian-Zun Tao;;Decreased proliferation ability and differentiation potential of mesenchymal stem cells of osteoporosis rat[J];Asian Pacific Journal of Tropical Medicine;2014年05期
15 鲍圣涌;;Effect of electroacupuncture on the bio-chemical indices of bone and bone collagen metabo-lism and TNF-α in osteoporosis model rats without ovaries[J];China Medical Abstracts(Internal Medicine);2013年01期
16 赖晓阳;;Effect of four different anti-osteoporosis agents on the expression of matrix gla protein in primary osteoblasts of SD rat[J];China Medical Abstracts(Internal Medicine);2013年01期
17 Paola Pisani;Maria Daniela Renna;Francesco Conversano;Ernesto Casciaro;Maurizio Muratore;Eugenio Quarta;Marco Di Paola;Sergio Casciaro;;Screening and early diagnosis of osteoporosis through X-ray and ultrasound based techniques[J];World Journal of Radiology;2013年11期
18 Jun Zhang;Ran Wang;Yi-Lei Zhao;Xiao-Hui Sun;Hong-Xing Zhao;Tan Lu;De-Cai Chen;Hai-Bin Xu;;Efficacy of intravenous zoledronic acid in the prevention and treatment of osteoporosis:A meta-analysis[J];Asian Pacific Journal of Tropical Medicine;2012年09期
19 ;Effect of Tanshitone on prevention and treatment of retinoic acid-induced osteoporosis in mice[J];沈阳药科大学学报;2008年S1期
20 ;Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis[J];Journal of Nanjing Medical University;2007年05期
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