Experimental Eradication of Pinwonns ( Syphacia obvelata and Aspiculuris tetraptera ) from Mice Colonies using Ivennecfin

【摘要】：正 Because of the frequent, worldwide transfer of mice between laboratories and researchers, pinworm parasites like Syphacia obvelata and Aspiculuris tetraptera are relatively common in genetically altered mice such as transgenic or knockout mice. Although pinworm infection usually does not produce any clinical illness in normal mice, heavy worm burdens have been associated with rectal prolapse, intussusception, and intestinal impaction. In addition, immunocompromised mice, such as nude mice or SCID mice, are predisposed to large worm burdens, sequelae, and death. Since parasitized mice are not desirable as ex- perimental subjects, it is important to keep laboratory rodent colonies parasite free. Ivermectin has been reported to be an effective anti-parasite agent for safely eliminating endo-and ecto-parasite(s) from infected mice.Spray administration is reported to be the most effective and practical method of application. The aim of this study was to investigate the effectiveness of ivermectin spray on mice infected with pinworms under experimental conditions in a practical application , and to evaluate the safety of the drug on murine reproduction in large breeding colonies. A spray of 0.1 % ivermectin inject-able solution over the entire cage once a week, for three consecutive weeks (one cycle treatment) , was effective in eradicating both Syphacia obvelata and Aspiculuris tetraptera from mice under experimental conditions. In addition, no acute toxicity was observed in 105 mothers or 687 neonates treated with ivermectin, indicating that ivermectin does not affect murine reproduction. Finally , we attempted to eradicate pinworms from infected mice in our institute using this method. Two cycles of treatment were administered , with a two-week pause between cycles, resulting in complete eradication for at least one year. The results of this study show that the spray administration of ivermectin used in the present protocol is safe, economical, requires very little labor and highly effective treatment for eradicating S . obvelata and A . tetraptera infectior.