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Anti-inflammatory Effects of Liuwei Dihuang Wan on Aβ25-35-induced BV2 cells

Junying Song  Yong YUAN  Ya-quan JIA  Zi-chuang WANG  Zhen-qiang ZHANG  
【摘要】:Objective: The β-amyloid protein(Aβ)-induced neurotoxicity is considered to be the main causative factor for Alzheimer's disease(AD).Liuwei Dihuang Wan(LDW) is popular Chinese herbal formula, which is described as an anti-inflammatory prescription in ancient Chinese herbal books.In this study, we investigated the inhibitory effects of LDW drug serum on Aβ_(25-35)-induced nervous inflammation in BV-2 cells and the related inflammatory pathways.Methods: The serum of mice was prepared by the method of serum pharmacology of traditional Chinese medicine.The control group, Aβ group and Aβ+ serum group were set up.The effect of LDW on BV-2 cell activity was determined by MTT.The expressiones of TBP2 and NLRP3 in the cells of different treatment groups were determined by Quantitative Real-time PCR and Western blot.Immunofluorescence used to localize the TBP2 and NLRP3 antigen in cells.Results:(1) LDW drug serum significantly inhibited Aβ_(25-35)-induced toxicity;(2) LDW drug serum attenuated the mRNA of TBP2 and NLRP-3 which were induced by Aβ_(25-35);(3) LDW drug serum decreased Aβ_(25-35)-induced expression of TBP2and NLRP3;(4) The fluorescence intensity of LDW drug serum group was lower than control group.Conclusion: These data suggest that LDW could protect BV-2 cells against Aβ_(25-35)-induced nervous inflammation through enhancing intracellular antioxidant activity and regulating TBP2 and NLRP3 mediated signal pathway.LDW might be a potentially therapeutic agent for AD treatment.

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