Expression and Significance of survivin in Oral Squamous Cell Carcinoma
【摘要】：正Background:Survivin is a novel member of the IAP family.Survivin in most adult normal tissues is undetectable, whereas its expression in human common tumors has been associated with increased aggressiveness and decreased patient survival,suggesting that apoptosis inhibition by survivin may be an important predictive/prognostic parameter of poor outcome in human cancers. Objective:To investigate the expression of survivin protein and survivin mRNA in oral squamous cell carcinoma(OSCC),try to find a new way to diagnose OSCC non-invasively,and investigate the roles of survivin in the development of OSCC.Methods:21 patients with OSCC were studied and 10 patients with benign disease were served as control.Saliva,oral exfoliate cells and serum were collected from every patient before and after operation.Specimens of Tumors with patient-matched normal epithelium were obtained in operation. The protein contents of survivin in all samples were quantitatively determined by enzyme-linked immunosorbent assay(ELISA). The expression of survivin protein in the tissues was examined by Immunohistochemistry.The expression of survivin mRNA was determined by RT-PCR and in situ hybridization(ISH).Results:The protein contents of survivin in exfoliate cells of OSCC(43.93±33.30 pg/ml) were significantly higher than the result in control group(8.49±3.73 pg/ml)(P0.01),but there was no significant difference in saliva and serum;The survivin protein contents correlated with lymph nodes metastasis(P0.05);Survivin mRNA was expressed in 92.3%of OSCC,most cases revealed a survivin-immunoreaction in the cytoplasm of the tumor cells;While it was expressed in 15.4% of neighboring normal tissues,the disparity was statistically significant(P0.01).The expression of survivin mRNA and survivin protein did not correlated with gender of patient,location,differentiation,TNM staging of tumor(all P0.05).Conclusion:survivin may be potentially contribute to the carcinogenesis of OSCC.Detection of survivin in oral exfoliate cells may be a non-invasive method in the diagnosis and prognosis of OSCC.