Quantitative analysis of miRNA expression in several developmental stages of human livers
【摘要】：正Background:miRNAs have been found to regulate gene expression in cells.There are studies showing that expression of miRNAs is tissue-specific and developmental stage-specific.The mechanism behind this might be the actions of the miRNAs. Methods:We introduce the identification of miRNAs from two human fetal liver cDNA libraries by a cloning protocol.Then the miRNAs detected were analyzed in a chorionic villus tissue and 4 liver tissues by using real-time PCR. Results:After sequencing and database searching,totally 42 miRNAs in two fetal livers were detected.Quantitative analysis showed that they have higher expression in the livers of the fetal period than the adult period,and furthermore the expression levels of the miRNAs during the fetal period was dynamically changed. Conclusion:Our results indicate that a special group of miRNAs may play an important role in human fetal liver development,and their roles in the adult livers are limited.