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In vitro and in vivo studies evaluating antisemen antibodies as a potential spermicidal agent in hamsters

【摘要】:正Objective:To determine the spermicidal activity of antisemen antibodies in the hamster model. Design:Prospective,controlled study. Setting:Advanced preclinical sciences center. Animal(s):Subgroups of 10 and 14 golden hamsters. Intervention(s):Ex vitro and in vivo treatment of sperm with antisemen antibodies or normal rabbit serum. Main Outcome Measure(s):The EC_(50) value of antisemen antibodies,the time required for 50%motility loss of progressively motile spermatozoa exposed to antisemen antibodies,the average sperm mitochondrion fluorescence intensity,the rate of fertilization,and the scoring of histologic changes in the hamster vaginal tissue. Restllt(s):The EC_(50) value of antisemen antibodies was found 70μg/mL,and the time required for 50%motility loss of progressively motile spermatozoa exposed to antisemen antibodies(at 70μg/mL) was 5 minutes;for the experimental and control groups,the average fluorescence intensities of sperm mitochondria were respectively 180.28±82.24 and 309.74±148.37,the fertilization rates in vitro were 0.09%and 45%,the rates of fertilization with intrauterine sperm injection were 0 and 15.0%.There was a significant difference between two groups.None of the four hamsters that received antisemen antibodies in gel-poiyoxyl-40-stearate had epithelial disruption characteristic of inflammation. Conclusion(s):Antisemen antibodies possess appreciable spermicidal potential,which may be explored as an effective constituent of spermicide.(Fertil Steril~? 2009;92:1116-23.?2009 by American Society for Reproductive Medicine.)

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