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De-novo assembly and characterization of Chlorella minutissima UTEX2341 transcriptome by paired-end sequencing

Mingjia Yu  Xiangzhi Lin  Shanjun Yang  Zhaokai Wang  Yang Zhang  
【摘要】:Chlorella minutissima is considered as one promising feedstock for biofuels in the future. Here, the transcriptome from oil-rich strain UTEX2341 of C. minutissima was generated based on Illumina paired-end sequencing. Through de-novo assembly, 14,905 total isogenes was obtained and compacted into 6,216 unigenes. 80% of unigenes were assigned with GO terms and further subdivided into 55 sub-categories. KEGG analysis showed that 37.2% of unigenes could be accessed and mapped into 278 pathways. Interestingly, genes encoding key enzymes involved in biosynthesis, elongation, and metabolism of fatty acids were identified, including malonyl-Co A-ACP transacylase, 3-ketoacyl-ACP synthase, 3-ketoacyl-ACP reductase, and others. Moreover, the genes involved in triacylglycerol(TAG) biosynthesis and metabolism also were found. Therefore, the transcriptome analysis of C. minutissima UTEX2341 not only supplies a comprehensive insight into the molecular pathway involved in biosynthesis of biofuels precursors, but also provides substantial valuable genomic resources to accelerate further development and utilization of biofuels.

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