Proteomic Analysis of Human Epithelial Ovarian Cancer Xenografts in Immunodeficient Mice Exposed to Chronic Psychological Stress
【摘要】：To compare alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice. Aslo, to explore the molecular mechanisms linking chronic psychological stress to ovarian cancer oncogenesis and progression. Methods: SK-OV-3 cells were injected subcutaneously into nude mice. The stress group was subjected to a chronic physical restraint protocol for 6 h on 35 consecutive days, while the control group was unrestrained. All mice were sacrificed on Day 36 after SK-OV-3 cell injection, and tumors were excised. Tumor tissues were processed for 2D gel electrophoresis, mass spectrometry (nanoUPLC-ESI-MS/MS) and western blotting. Results: The expression of twenty proteins was found to be significantly altered between the stress and control groups, of which fourteen were up-regulated, five were down-regulated, and one protein was found only in the stress group. All proteins were identified by UPLC-ESI-MS/MS, and western blotting results were consistent with those of proteomic methods. Conclusions: The present results provide new evidence relating to the molecular mechanism underlying the relationship between psychological stress and tumor progression.