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The Toxic Effects of Alveolar Macrophage(AM) Induced by Three Typical Nanomaterials

【摘要】:[Objection] To study the toxic effects of alveolar macrophages(AMs) induced by three typical nanomaterials and explore the relationship between the nanoparticles properties and the toxic response of AM treated in vitro with these different nanoparticles: silver nanoparticles (Nano-Ag), zinc oxide nanoparticles(Nano-ZnO)and titanium dioxide nanoparticles (Nano-TiO 2 ). [Method] In this study, the particles were suspended in fetal bovine serum, which was found to be the best dispersing vehicle in our pilot study and also used by other researchers. The issues of dose exposure are appropriate for toxicological studies have been addressed. AM cells were freshly derived and exposed to various doses (2.5,5,10,25,50,100 and 200μg/ml) of three nanoparticles. Cytotoxicity was sufficiently measured by WST assay, lactate dehydrogenase (LDH) assay. The effect of AMs phagocytic function induced by nanoparticles were measured by neutral red phagocytosis assay. [Result] The findings of the study showed that three nanomaterials can lead to decrease activity of AM cells and affect cell membrane integrity and permeability to inhibite cell activity. AM cells phagocytic function were significantly reduced by nanoparticles, weakening the nonspecific defense capability. The toxic effect comparisions amid three nanomaterials were distinct: the Nano-ZnO induced the most significant toxic while the Nano-TiO 2 induced the laest. [Conclusion] Particle composition and shape probably played a primary role in the toxic effects of different particles and the findings are of sufficient significance to warrant further mechanism studies.

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