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New insights into the evolution and functional divergence of the CIPK gene family in Saccharum

苏炜华  Yongjuan Ren  Dongjiao Wang  Long Huang  Hui Ling  Yachun Su  Ning Huang  Hanchen Tang  Liping Xu  Youxiong Que  
【摘要】:【Research background】 CBL-interacting protein kinases(CIPKs) are the primary components of calcium sensors and play crucial roles in plant developmental processes, hormone signaling transduction, and in the response to exogenous stresses. Sugarcane is an important sugar crop that contributes up to about 80% of the world sugar production. To date, CIPK genes have not been investigated in sugarcane. 【Materials and methods】 In this study, sequence and evolution analysis of the SsCIPK genes were conducted using the available sugarcane genome data. In addition, the expression patterns of the CIPK gene family in the presence of abscisic acid(ABA), polyethylene glycol(PEG), and sodium chloride(NaCl) were detected by qRT-PCR. The allogenic expression of ScCIPKs and interactions between ScCIPKs and ScCBLs were also explored. Under salinity stress, the growth status of E. coli cells expressing ScCIPKs was analyzed. Furthermore, their subcellular localization, a bimolecular fluorescence complementation assay(BiFC), and transient overexpression in Nicotiana benthamiana were also investigated.【Result and analysis】 In this study, 48 CIPK genes(Ss CIPKs) were identified from the genome of Saccharum spontaneum. Phylogenetic reconstruction suggested that the SsCIPK gene family may have undergone six gene duplication events from the last common ancestor(LCA) of Ss CIPKs. The gene structure of Ss CIPKs also incurred substantial variations during evolution, with the number of introns varying from 0 to 15. Whole-genome duplications(WGDs) and signal-gene replications served as the driving force for the amplification of SsCIPKs. Ten Sc CIPK genes were amplified from sugarcane(Saccharum spp. hybrids). Expression analyses of ScCIPK genes using qRT-PCR revealed that ScCIPKs are differentially expressed in response to ABA, PEG, and NaCl. Subcellular localization of ScCIPKs revealed that the fusion protein of four ScCIPK::GFP were all expressed in the cytoplasm and plasma membrane, except for ScCIPK17::GFP, which has also been observed in the nucleus. Prokaryotic expression implied that the recombinant proteins of ScCIPK3,-15, and-17 could only slightly enhance growth under NaCl stress conditions, but ScCIPK21 did not. Transient N. benthamiana plants overexpressing ScCIPKs demonstrated that the ScCIPK genes are involved in responding to external stressors through the ethylene synthesis pathway as well as to bacterial infections. Preferential interactions between ScCBLs and Sc CIPKs were observed in BiFC experiments. 【Conclusion】 These results improve our understanding of the evolution and functions of the CIPK gene family in sugarcane complexes as well as provide a basis for selecting appropriate genes for in-depth functional studies of CBL-CIPK in sugarcane.

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1 Jianle LIU;Changjun BAI;Linling YAN;;Energy Productivity Potential Assessment of Saccharum spontaneum L. Germplasm Resources[J];Agricultural Science & Technology;2014年12期
2 Jianle LIU;Changjun BAI;Linling YAN;Shimeng CHEN;Yu ZHANG;Hubiao YANG;;Nutritional Value Evaluation of Saccharum spontaneum L. Germplasm Resources[J];Agricultural Science & Technology;2014年11期
3 王丽萍;蔡青;范源洪;陆鑫;Karen Aiken;马丽;刘新龙;夏红明;;甘蔗(Saccharum)与斑茅(Erianthus arundinaceus)远缘杂交利用研究[J];西南农业学报;2007年04期
4 Vikas Y Patade;P Suprasanna;V A Bapat;;Gamma Irradiation of Embryogenic Callus Cultures and In vitro Selection for Salt Tolerance in Sugarcane (Saccharum officinarum L.)[J];Agricultural Sciences in China;2008年09期
5 邝炎华,刘琼英,郑粤美,邓兆活,徐声杰;稀土元素在甘蔗(Saccharum officinarum L.)体内运转、分配及其对磷、钾素吸收的影响[J];核农学报;1991年03期
6 Hongbo LOU;Xianhong WANG;Lilian HE;Fusheng LI;;ISSR Polymorphism Analysis of Eight F_1 Hybrids of Erianthus fulvus and Saccharum spp.[J];Agricultural Biotechnology;2012年04期
7 ;Analysis of Disequilibrium Hybridization in Hybrid and Backcross Progenies of Saccharum officinarum × Erianthus arundinaceus[J];Agricultural Sciences in China;2010年09期
8 Jiale JI;Qinnan WANG;Yongsheng QIU;Cheng FU;Qiwei WU;Houxiang HU;Feng ZHOU;Chuiming ZHANG;Huanying XU;Jing XIE;;Genetic Research and Utilization Reference of Saccharum spontaneum L.[J];Agricultural Biotechnology;2019年03期
9 Xin Zhang;Yongguan Zhu;Yuebin Zhang;Yunxia Liu;Shaochun Liu;Jiawen Guo;Rudan Li;Songlin Wu;Baodong Chen;;Growth and metal uptake of energy sugarcane (Saccharum spp.) in different metal mine tailings with soil amendments[J];Journal of Environmental Sciences;2014年05期
10 李鸣,谭裕模,李杨瑞,李容柏,高国庆;甘蔗(Saccharum officinarum L.)品种遗传差异的AFLP分子标记分析[J];作物学报;2004年10期
11 Mingfu WEN;Junxian YANG;Fangyin PAN;Wenlong WU;Yuegui CHEN;Jinyan GUAN;;Advances in Studies of Genetic Improvement of Sugarcane[J];Asian Agricultural Research;2016年11期
12 Ashok A.Nikam;Rachayya M. Devarumath;Akash Ahuja;Harinath Babu;Mahadeo G.Shitole;Penna Suprasanna;;Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane(Saccharum officinarum L.)[J];The Crop Journal;2015年01期
13 李鸣,谭裕模,李杨瑞,李容柏,高国庆;用AFLP分子标记技术鉴别甘蔗(Saccharum of ficinarum L.)品种[J];分子植物育种;2003年Z1期
14 林国栋,陈如凯;甘蔗的分类──I·Saccharum的近缘植物[J];甘蔗;1995年01期
15 Jianle LIU;Changjun BAI;Linling YAN;Qinglin JIA;Yu ZHANG;Hubiao YANG;;Study on Saccharification of Saccharum spontaneum L. by Sulphuric Acid Hydrolysis[J];Agricultural Biotechnology;2014年06期
16 宋顺喜;郝宁;;添加尿素的绿色除硅技术[J];国际造纸;2013年04期
17 刘树楷;郑建仙;;古老的人工甜味剂——糖精(Saccharin)[J];甜菜糖业;1991年01期
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