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Transcription profile analysis of interaction mechanism between sugarcane and Sporisorium scitamineum using microarray

黄宁  Hui Ling  Weihua Su  Yachun Su  Youxiong Que  
【摘要】:【Research background】 Sugarcane production is seriously affected by Sporisorium scitamineum infection in the world. Although some excellent resistant breeding materials have been created, the utilization of sugarcane germplasm and the breeding of resistant varieties are still greatly restricted for the complex genetic background of sugarcane and the differentiation of smut race. To solve this problem, much effort should be devoted to underlying the mechanism of sugarcane smut resistance and S. scitamineum virulence. 【Materials and methods】 According to our previous RNA-seq and smut pathogen genome data, the gene expression chip was customized. The transcription profile of gene set, which referred to 2,0392 from sugarcane and the whole CDS sequence(6,621) from S. scitamineum genome, was identified on smut spore injected samples. These samples were from sugarcane smut-susceptible genotype YC71-374 and smut-resistant genotype NCo376. 【Result and analysis】 During the interaction between sugarcane and smut fungus, 6,842 sugarcane genes and 3,569 S. scitamineum genes were differentially expressed. According to the GO and KEGG enrichment analysis, the differential expressed sugarcane genes involved in primary metabolism pathway, MAPK pathway, plant hormone signal transduction pathway and ROS response, the differential expressed S. scitamineum genes mostly located in vesicles, and have activities like transportation, signal transduction and redox. After that, the differential expressed genes from sugarcane and S. scitamineum were selected and used to construct the weighted gene co-expression(WGCNA) network of sugarcane-S. scitamineum interaction. Finally, two sugarcane smut-resistance related genes(vacuolar-sorting receptor, ScVSR; and leucine-rich repeat receptor-like protein kinase, Sc LRR-RLK) and four putative S. scitamineum effector genes, including protein essential during penetration 1(SsPep1), hypothetical cysteine-protease inhibitor(SsE4), conserved hypothetical protein SsE11 and hypothetical protein SsE11, were selected from the WGCNA network. 【Conclusion】 This study aims to understand the molecular mechanism of sugarcane-S. scitamineum interaction by the characterization of gene transcription and gene function, as well as to identify gene related to the proliferation of smut fungus, mine pathogenesis-related genes and smut effectors. It should help to pave the way to the conventional breeding for smut resistance in sugarcane.

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