Effect of L-carnitin on Cadmium Induced Toxicity in Rat Embryo Hippocampus

【摘要】：Cadmium is a toxic metal which is widely used in industry.This metal exerts toxic effects on multiple organs,including nervous system.The aim of this study have been evaluated the effect of cadmium on weight and development of hippocampus in Wistar rat embryos and then determining if L-carnitin,as an antioxidant,could protect hippocampus from the toxic effects.Female Wistar rats(250-300 g) were used in this study.24 hours after mating with male rats,the females were separated and their vaginal smears were obtained for sperm detection.This day was considered as embryonic zero day.The females were divided into three groups:Control group which received no injection,Experimental groups which received 1mg/kg B.W cadmium or 1mg/kg B.W cadmium + 500mg/kg B.W L-carnitin in days 7 and 10 of gestation.On day 17 of gestation,the animals were sacrificed by chloroform over dose and their embryos were removed surgically.The embryos were fixed in formalin 10% for 30 days.Then weight of embryos was measured and tissue processing,sectioning and hematoxylin-eosin(HM) staining were done.Some sections of hippocampus were evaluated using light microscope and MOTIC software.The weights of embryos were significantly decreased in experimental groups.This decrease was significantly greater in the first experimental group.The number of cells and thickness of hippocampus layers were decreased significantly just in the first group.These findings indicate that cadmium has teratogenic effects on embryo's weight and development of hippocampus and at least a part of these effects may be inhibited by L-carnitin.