收藏本站
收藏 | 手机打开
二维码
手机客户端打开本文

Non-target-site and target-site resistance of Beckmannia syzigachne Steud.to AHAS inhibitors in China

Jingjing Wang  Xiangju Li  Huilin Yu  Hailan Cui  
【摘要】:American sloughgrass(BeckmanniasyzigachneSteud.) is one of the most competitive and malignant weeds in rice-wheat rotation areas of China.American sloughgrasswere not sensitiveto Acetohydroxyacid synthase(AHAS) inhibitors.There were two resistant American sloughgrass populations(R1 and R2) showed resistance to inhibitors of AHAS by using whole-plant dose-response assay,DNA sequencing and in vitro AHAS enzyme assay.Whole-plant dose-response assay indicated that Rl showed moderately resistant to mesosulfuron-methyl(3.3-fold),flazasulfuron(4.7-fold),imazapic(2.8-fold),pyroxsulam(1.6-fold) and pyribenzoxim(7.4-fold),and highly resistant to tlucarbazone(11.7-fold).However,using malathion which is a cytochrome P450 inhibitors combined with mesosulfuron-methyl could reduce the level resistance of Rl.Furthermore,R2 was highly resistant to flazasulfuron(34.9-fold),imazapic(11.3-fold),flucarbazone(49.2-fold),pyribenzoxim(12.9-fold),moderately resistant to mesosulfuron-methyl(9.8-fold) and pyroxsulam(6.3-fold).Amplified partial AHAS gene was sequenced and in vitro AHAS enzyme was assayed in order to clarify the resistance mechanism of American sloughgrass populations.The results showed that R2 had Pro-197-Ser mutation in the AHAS genewhich resulted in the R2 resistance to AHAS inhibitors.There was no mutation in AHAS gene in Rl and also no significant differences in enzyme sensitivity between the susceptible(S) and resistant population(Rl),enhanced the metabolism may be the main mechanism of Rl resistance to AHAS inhibitors.

知网文化
【相似文献】
中国期刊全文数据库 前4条
1 ;Influence of Organic Manure on Organic Phosphorus Fraction in Soils[J];Pedosphere;1993年04期
2 ;RESULTS OF THE ZOOLOGICO-BOTANICAL EXPEDITION OF ACADEMIA SINICA AND ACADEMY OF SCIENCES USSR TO SOUTHWEST CHINA 1955--1957(COLEOPTERA, LANGURIIDAE)[J];昆虫学报;1959年03期
3 ;ON SOME NEW AND RARE DENTALIUM FROM CHINA COASTS[J];中国动物学杂志;1950年00期
4 ;Evaluation of Rice Germplasm for Resistance to the Small Brown Planthopper (Laodelphax striatellus) and Analysis of Resistance Mechanism[J];Rice Science;2008年01期
中国重要会议论文全文数据库 前5条
1 ;Development of a gene cloning system in fast-growing and moderately-thermophilic Streptomyces and heterologous expression of Streptomyces antibiotics biosynthetic gene clusters[A];2010年第四届全国微生物遗传学学术研讨会论文摘要集[C];2010年
2 T.Shan;J.D.Zhao;;Pressure Dip of Conical Sand Pile Formed in Water[A];Proceedings of the 16th Annual Conference of Hong Kong Society of Theoretical and Applied Mechanics 2012、The 1st Mainland-Hong Kong Youth Forum on Mechanics 2012、The 8th Shanghai-Hong Kong Forum on Mechanics and Its Application 2012[C];2012年
3 张旻;梁毅;;Cytotoxic aggregates of α-lactalbumin induced by unsaturated fatty acid induce apoptosis in tumor cells[A];第二届全国“跨学科蛋白质研究”学术讨论会论文集[C];2008年
4 ;Biological hydrogen production from corn stover by moderately thermophilic bacteria[A];2008 International Hydrogen Forum Programme and Abstract[C];2008年
5 ;Cellular Communication-Induced Dynamics in a Multicellular System[A];中国自动化学会控制理论专业委员会B卷[C];2011年
中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978