The dynamics of shikimate accumulation and chlorophyll relative content in Calystegia hederacea Wall.following glyphosate application

【摘要】：To clear the dynamics of shikimate accumulation and chlorophyll relative content in C.hederacea Wall,with two growth stage after glyphosate application.Shikimate accumulation and chlorophyll relative content was determined by spectrophotometric assay and the SPAD-502 readings,respectively.Shikimate accumulation levels keep declining at the dose of 410 g a.i./ha.The SPAD values declined to 42 then decreased to the control levels.The amount of shikimate accumulation in the elder plants decreased to 2145.3 μg/g at 4 days then declined to 907.1μg/g after 1640 g a.i./ha glyphosate application and the SPAD values declined to 38.3 then increased slightly.The amount of shikimate accumulation in the young plants keep increasing to 2258.2μg/g at this dosage while the relative chlorophyll content in the leaves of young plants keep declining after treatment which the SPAD values was 29.4 at 7 days after treatment,it is 0.56 fold lower than the control levels.All the shikimate and chlorophyll can reflect the sensitive of C.hederacea to the glyphosate.This result can use for developing the methods to investigative the grass that are naturally resistant to glyphosate.