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《浙江省第四届动物学博士与教授论坛、动物学与经济强省-浙江省动物学研究及发展战略研讨会论文摘要集》2017年
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Indole reduce the expression of virulence related gene of Vibrio splendidus pathogenic to sea cucumber Apostichopus japonicus

Shanshan Zhang  Ningning Liu  Tongxiang Song  Huijie Liu  Weiwei Zhang  Chenghua Li  
【摘要】:Indole is a metabolite of tryptophan that can be synthesized by various bacteria.In the present study,production of indole by Vibrio splendidus Vs was determined using Kovac's reagent,and m/z was further determined by HPLC-MS.Extracellular indole reached a maximum concentration of 160 μM,when OD_(600)of V.splendidus Vs was approximately 0.9.In addition,glucose could reduce indole level,and 1%(m/v) glucose could reduce the mRNA level of tnaA,the gene enconding tryptophanase,down to 0.2%.To investigate the effects of indole on the mRNA levels of virulence related genes of V.splendidus Vs,mRNA levels of vsm,vsh and ABC respectively related to protease activity,haemolytic activity and ABC transporter ATP-binding protein were determined.Exogenous indole supplemented at a concentration of 125 uM could respectively down regulate the mRNA level of vsm,vsh and ABC to 9,7 and 11%.Meanwhile,indole could alter the expressions of immune related gene in Apostichopus japonicus.When coelomocytes were co-cultured with exogenous indole at a concentration of 125 μM,the mRNA level of AjLBP/BP and Ajp105,were up regulated by 1.6-fold and 1.4-fold,respectively.Combined all the results in our study suggested that indole could alter the expressions of both virulence related genes in pathogenic V.splendidus Vs and the immune related genes in A.japonicus.
【作者单位】:School of Marine Sciences,Ningbo University
【分类号】:S917.4

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